南方鲇CART基因cDNA克隆分析及组织分布  

Cloning, Sequence Analysis and Tissue Distribution of CARTGene cDNA fromSilurus meridionalis

作　　者：彭焕文[1] 杨雁[1] 尹传龙[1] 李艳利[1] 张昊星[1] 

机构地区：[1]西南大学淡水鱼类资源与生殖发育教育部重点实验室,重厌400715

出　　处：《重庆师范大学学报（自然科学版）》2013年第3期21-27,F0003,共8页Journal of Chongqing Normal University:Natural Science

基　　金：重庆市自然科学基金(No.CSTC2008BB1098);国家重点基础研究发展计划资助(No.2010CB134405);重庆市科委重点实验室专项经费资助(2012)

摘　　要：采用RT-PCR和RACE技术克隆得到南方鲇(Silurus meridionalis)的CART基因的全长cDNA序列,全长688bp,其中5’非翻译区长109bp,ORF长357bp,3’非翻译区222bp,编码118个氨基酸。与其他脊椎动物的CART蛋白氨基酸序列比对发现,南方鲇CART与斑点叉尾鮰(Ietalurus punetaus)CART、金鱼(Carassius auratus)CART1、金鱼CART2、鲤鱼(Cyprinus carpio)CART1、鲤鱼CART2和人(Homo sapiens)CART同源性分别达到95.8%、72.0%、75.0%、72.9%、75.0%和52.5%,表现出较高的保守性。进化树结果显示,南方鲇的CART与斑点叉尾鮰聚为一支,与基于形态学的鱼类系统发育结论相吻合。组织分布分析表明南方鲇CART基因mRNA主要在脑中表达,与之功能相吻合。研究结果为该种鱼摄食调控机制的研究提供了新的基础资料。The full length cDNA of southern catfish CART （cocaine-and amphetamine-regulated transcript） was cloned RNA by RT- PCR and RACE. It contains 688 nucleotides, including 5＇ UTR of 109 nucleotides, 3＇ UTR of 222 nucleotides and an open reading frame （ORF） with 357 nucleotides encoding a 118-amino acid peptide. The results showed that CART of southern catfish has 95.8%, 72.0%, 75.0%, 72.9%, 75.0% and 52.5% similarity with CART protein from channel catfish, goldfish, goldfish 2, common carp 1, common carp 2 and human, respectively. CART protein of southern catfish was clustered closely with channel cat- fish, in accordance with the phylogeny of fishes. The result of tissue distribution showed that CART gene mRNA was mainly ex- pressed in the brain, in accordance with CART＇ s function. Our results provide new basic data for feeding-control mechanism in this fish.

关 键 词：南方鲇 CART基因 克隆 分析 MRNA 组织分布 

分 类 号：Q959.4[生物学—动物学] Q785