常规降糖与联合N-乙酰半胱氨酸对糖尿病慢性肾脏疾病患者PARP-1及GAPDH活性影响的研究  

Effect of glucose-control and N-Acetylcysteine on PARP and GAPDH activities in diabetic patients with chronic kidney disease in diabetes

作　　者：朱恒梅[1] 祝胜郎[1] 李向阳[1] 陈结慧[1] 蒋莹[1] 叶玲[1] 

机构地区：[1]广东医学院附属南山医院肾内科,深圳518052

出　　处：《中国糖尿病杂志》2013年第5期426-428,共3页Chinese Journal of Diabetes

基　　金：2011年深圳市科技局科技立项(201102134);2011年深圳市南山区科技局基金(南卫2011004)

摘　　要：目的观察采用常规降糖治疗与联合N-乙酰半胱氨酸(NAC)治疗对糖尿病慢性肾脏病(CKD)患者多聚ADP核糖聚合酶(PARP)、3-磷酸甘油脱氢酶(GAPDH)活性的影响。方法将60例CKDⅢ期患者随机分为常规治疗组(A组)30例;联合NAC治疗组(B组)30例;另选取同期健康体检者30名为正常对照(NC)组,治疗12周。观察治疗前后外周单核细胞PARP活性、全血GAPDH活性的变化。结果 A、B组治疗后PARP活性下降[A组(3.70±0.06)vs(3.02±0.07)U/mg;B组(3.74±0.11)vs(2.66±0.04)U/mg],GAPDH活性升高[A组(4430.8±279.4)vs(5136.6±321.6)U/ml,B组(4495.2±324.7)vs(6104.4±275.3)U/ml],且B组治疗后PARP及GAPDH活性改变与A组比较,差异有统计学意义(P<0.05)。结论联合NAC治疗与常规治疗相比,可更有效地逆转CKD患者PARP、GAPDH活性的异常。Objectives To observe the effects of conventional treatment and N-Acetylcysteine （NAC） on the activities of PARP and GAPDH in the patients with diabetic nephropathy. Methods A total of 60 patients with DN （stage Ⅲ） were included and randomized into two groups. The conventional group （30 cases） was treated with conventional treatment, and the NAC group （30 cases） was treated with NAC plus conventional treatment. Another 30 healthy people were recruited as controls. PARP activity in peripheral blood mononuclear cells, GAPDH activity in whole blood before and after 12 weeks＇ treatment were measured. Results 1. After treatment, the PARP activity in both groups was decreased and GAPDH activity increased （P〈0.05） [conventional group （4430.8±279.4） vs （5136.6±321.6）U/ml,NAC group （4495.2±324.7） vs （6104.4±275.3）U/ml]. 2. Changes of PARP and GAPDH activities after treatment in NAC group were more significant （P〈0.05）. Conclusion Compared with the conventional treatment, the NAC plus conventional treatment can reverse disorders of PARP and GAPDH activities in the DN patients more effectively.

关 键 词：糖尿病慢性肾脏疾病 多聚ADP核糖聚合酶 3-磷酸甘油脱氢酶 

分 类 号：R692[医药卫生—泌尿科学]