樱桃谷鸭人工感染鸭疫里默杆菌肝脏差异基因的筛选与鉴定  

The difference gene screening and identificakion of liver in cherry valley duck artificially anatipestifer

作　　者：朱洪云[1] 陈志虎[2] 

机构地区：[1]西藏农牧学院动物科学学院,西藏林芝860000 [2]华中农业大学兽医学院,湖北武汉430070

出　　处：《中国兽医学报》2013年第5期675-679,共5页Chinese Journal of Veterinary Science

摘　　要：鸭疫里默杆菌病给全世界养鸭业带来了严重的经济损失,但人们对其毒力因子以及致病的分子机理,尤其是宿主对感染的应答反应还缺乏深入认识。本研究利用抑制消减杂交技术(SSH)构建了鸭疫里默杆菌感染过程中鸭肝脏组织差减cDNA文库,通过PCR和斑点杂交筛选到45个在鸭疫里默杆菌感染过程中鸭肝脏组织差异表达的基因,这些基因的功能分为急性期反应、炎症反应、免疫和防御应答、损伤修复以及其他功能。利用real-timeRT-PCR对其中20个基因的在感染过程中不同时间段(8、24和48h)表达水平进行了验证,结果表明这些基因在鸭疫里默杆菌感染过程中表达水平都有不同程度的上调或下调(P<0.05或P<0.01)。试验结果表明,鸭疫里默杆菌感染引起鸭肝脏的一系列反应,包括急性期反应、炎症反应、免疫应答以及损伤修复等,通过对这些基因的差异调节,一方面维持机体的内环境平衡状态,另一方面启动机体的免疫系统识别和抵抗病原菌的侵袭。The molecular mechanisms of the disease is poorly understood, particularly the host re- sponse to R. anatipestifer infection. The differential expressed gene profile of the duck liver during R. anatipestifer infection was therefore investigated by using the suppression subtractive hybridizaton （SSH） analysis. A total of 45 differential expressed genes that encoded proteins involved in acute phase response, inflammatory response, immune response, wound healing and others functions were identified by PCR and dot blotting. The expression levels of twenty genes at 8, 24 and 48 h were verified by real-time RT-PCR. All were differentially expressed following R. anatipestifer infection （P〈0.05 or P〈0.01） ,which suggests that a series of physiological reactions （acute phase response, inflammatory response, immune response, wound healing etc. ） were induced after the infection of RA to maintain the homeostasis,and to activate the immune system to recognize and defend pathogens.

关 键 词：樱桃谷鸭 鸭疫里默杆菌 肝脏 抑制性消减杂交技术 基因表达 

分 类 号：S852.61[农业科学—基础兽医学]