阿替美唑对小型猪特异性麻醉剂诱导大鼠大脑皮质c-jun蛋白表达的影响  

Effect of atipamezole on the specificity anesthetic for miniaturepigs induced c-jun expression in the rat cerebral cortex

作　　者：尹柏双[1,2] 李晓波[1] 石星星[1] 李志强[1] 张建涛[1] 高利[1] 王洪斌[1] 

机构地区：[1]东北农业大学动物医学学院,黑龙江哈尔滨150030 [2]吉林农业科技学院动物医学学院,吉林吉林市132101

出　　处：《中国兽医学报》2013年第5期746-749,共4页Chinese Journal of Veterinary Science

基　　金：国家自然科学基金资助项目(30671552;30972227;31272617);长白山动植物资源利用与保护省级重点实验室基金项目(吉农科合字2012701);吉林农业科技学院博士科研启动基金项目

摘　　要：研究阿替美唑对小型猪特异性麻醉剂(XFM)麻醉下大鼠大脑皮质c-jun蛋白表达的影响,探讨阿替美唑颉颃XFM催醒大鼠与脑皮质c-jun基因的关系。将78只SD纯种大鼠随机分为XFM对照组、XFM+阿替美唑组、XFM+生理盐水组。采用蛋白质印迹法检测大脑皮质内c-jun蛋白表达量。结果表明,XFM麻醉大鼠大脑皮质内c-jun蛋白表达量逐渐增加,与给药前0min比较差异显著(P<0.01或P<0.05);XFM麻醉大鼠腹腔注射生理盐水,各时间点c-jun蛋白表达与对照组间比较无显著差异(P>0.05);阿替美唑注射后引起XFM麻醉大鼠大脑皮质c-jun蛋白表达减少,与XFM对照组比较差异显著(P<0.01或P<0.05)。结果提示,大脑皮质c-jun基因参与了阿替美唑颉颃XFM麻醉作用。阿替美唑抑制XFM诱导大鼠大脑皮质c-jun蛋白表达,可能是阿替美唑催醒XFM麻醉大鼠的重要机理之一。This experiment was to investigate effect of atipamezole on XFM induced c-jun expression in the rat cerebral cortex. 78 SD rats were divided randomly into XFM group,XFM add atipamezole group,XFM add saline group. The content of c-jun protein in cerebral cortex was measured by Western blot. The c-jun protein contents in the XFM anesthesia group were obviously increased as compared with 0 rain before XFM administrated （P;0.01 or P%0.05）. The c-jun protein contents did not obviously change after saline administrated as compared with control group （P〉0.05）. The c-jun protein contents was significantly decreased after atipamzole administrated as compared with control group （P〈0.05 or P〈0.01）. These results indicated that atipamezole palinesthesia action was concerned with c-jun oncogene in cerebral cortex. Atipamzole attenuates XFM induced c-jun expression in the rat cerebral cortex, which may be an important mechanism of atipamzole woke up anaesthetic rat by XFM.

关 键 词：阿替美唑 小型猪特异性麻醉剂 C-JUN基因 蛋白质印迹 

分 类 号：S859[农业科学—临床兽医学]