荧光定量分析猪睾丸组织中miR-34b的表达规律  

Analysis of miR-34b expression in swine testicular tissues by RT-PCR

作　　者：赵伟[1] 李传民[1] 高峰[1] 张晓军[1] 王大力[1] 白春燕[1] 孙博兴[1] 

机构地区：[1]吉林大学畜牧兽医学院,吉林长春130062

出　　处：《中国兽医学报》2013年第5期785-787,794,共4页Chinese Journal of Veterinary Science

基　　金：吉林省科技发展计划项目(20120208);吉林大学基本科研业务费项目

摘　　要：运用荧光定量分析方法检测军牧1号白猪不同发育阶段睾丸组织miRNA-34b的表达量差异,结果:miRNA-34b在3月龄前的表达量都非常低,从5月龄开始表达量呈明显上升,7月龄达到峰值后开始明显下降。在猪的发育过程中,miRNA-34b的表达趋势曲线呈先增后降。结果表明miRNA-34b可能与猪雄性生殖发育相关。MicroRNAs （miRNAs） are short （-22 nucleotides） non-coding RNA molecules that play important roles by repressing translation or cleaving RNA transcripts. Some reports showed miRNA-34b is an important regulator in germ cell differentiation process of male humans and mice. We detected the different expressions of miR-34h of testicular tissue by real time quantitative PCR （RT-PCR） in Junmu No. 1 white pig among the whole developmental stages. The results showed the expressions of miRNA 34b keep very low level before 3-month-old, rising dramatically from 5-month old,reaching a peak at 7 month old,and then descend apparently. Overviev the expression trend of miRNA-34b in the whole sexual development processes,it runs a rule that first rising and then descending. The conclusion is anticipated that miR 34b keep a high possibility correlation with sexual development of male pig.

关 键 词：miRNA-34b 军牧1号白猪 荧光定量 

分 类 号：Q78[生物学—分子生物学]