NGF对输卵管黏膜上皮细胞促性腺激素受体表达的影响  

NGF affects the expression of gonadotropin receptors in oviduct epithelial cells

作　　者：刘立文[1,2] 马永和[2] 李万宏[2] 王春强[2] 周虚[2] 

机构地区：[1]河北北方学院动物科技学院,河北张家口075131 [2]吉林大学畜牧兽医学院,吉林长春130062

出　　处：《中国兽医学报》2013年第5期795-798,共4页Chinese Journal of Veterinary Science

基　　金：十二五国家科技支撑计划项目(2011BAD19B02)

摘　　要：本研究以牛输卵管黏膜上皮细胞为研究对象,利用荧光定量PCR方法检测NGF和酪氨酸激酶抑制剂K252α对输卵管粘膜上皮细胞膜促性腺激素受体FSHR和LHRmRNA表达的影响。添加30、60、100μg/L NGF可以显著增加FSHR mRNA的表达,添加30和60μg/L的NGF同样增加LHR mRNA的表达。添加抑制剂K252α后,LHR和FSHR的表达量均比对照组显著下降。该结果说明NGF可以通过其受体调节输卵管黏膜上皮细胞促性腺激素受体FSHR和LHR的表达。We investigated the effects of NGF and K252a on the expression of gonadotropin recptors（FSHR and LHR） in cow oviduct epithelial cells with q-PCR. The expression level of FSHR in cells cultured media with NGF（30,60 or 100 μg/L） were significantly higher than that in the control. The expression level of LHR mRNA in cells cultured with NGF （30 or 60μg/L） were also significantly higher than that in the control. However,K252a could decreased the expression of FSHR and I.HR.

关 键 词：输卵管 神经营养因子 促性腺激素受体 

分 类 号：S852.1[农业科学—基础兽医学]