多指标正交试验优选白术芍药散提取工艺  

Optimization of extraction process for Baizhu Shaoyao San by multi-index orthogonal experiment

作　　者：郑礼娟[1,2] 秦昆明[1,2] 蔡皓[1] 曹岗[3] 蔡宝昌[1,2,3] 

机构地区：[1]南京中医药大学国家教育部中药炮制规范化及标准化工程研究中心,江苏南京210029 [2]南京海昌中药集团有限公司,江苏南京210061 [3]浙江中医药大学中药炮制技术研究中心,浙江杭州310053

出　　处：《中国中药杂志》2013年第10期1504-1509,共6页China Journal of Chinese Materia Medica

基　　金：国家自然科学基金项目(81202918);国家科技部火炬计划项目(2010GH020663);江苏省自然科学基金项目(BK2011135);南京市中药丸剂现代工程技术研究中心建设项目(GCZX2010051);南京市科技发展计划项目(201007005)

摘　　要：白术芍药散是中医传统名方,临床对于腹泻有确切的治疗效果。然而,目前有关白术芍药散的提取工艺报道较少。该试验利用正交试验的方法,以复方中10个指标性成分和浸出物的含量作为指标,采用综合加权评分法处理数据,对提取工艺中乙醇浓度、溶剂倍量、浸泡时间、提取时间4个因素进行考察,以优化白术芍药散提取工艺。结果得出白术芍药散的最佳提取工艺为加入12倍量70%乙醇,浸泡1 h,提取2次,每次2 h。其中提取时间有显著影响。优化后的提取工艺稳定可靠,可为进一步开发利用该处方提供参考。Baizhu Shaoyao San is a famous traditional Chinese medicine formula, which is efficient to diarrhea clinically. However, there are very few reports for extraction process of Baizhu Shaoyao San at present. In this article, an orthogonal test was conducted, with 10 major components and extractum of Baizhu Shaoyao San as indexes, and data was processed by the compound weight method, in order to detect four factors, including the concentration of ethanol, the dosage of ethanol, the soaking and extracting times, and optimize the extraction process of Baizhu Shaoyao San. The results showed that the optimal extraction process was adding 12-fold ethanol of 70% concentration, soaking for 1 hours, and extracting for twice, 2 hours each time. Among the indexes, the extracting time made significant impact. The optimized method is stable and reliable, and can provide reference for further development and utili- zation of the formula.

关 键 词：白术芍药散 正交试验 多指标 提取工艺 

分 类 号：R283[医药卫生—中药学]