狼毒(月腺大戟)不同炮制品的HPLC图谱及狼毒乙素、狼毒丙素、岩大戟内酯B的含量变化研究  

Study on HPLC chromatograms of different processed Euphorbia ebracteolata products and content change of three chemical components

作　　者：庄果[1] 李俊松[1,2] 蔡宝昌[1,2] 

机构地区：[1]南京中医药大学江苏省中药炮制重点实验室,江苏南京210046 [2]南京中医药大学中药学一级学科,江苏南京210046

出　　处：《中国中药杂志》2013年第10期1526-1530,共5页China Journal of Chinese Materia Medica

基　　金：国家公益性行业专项(200807039);南京中医药大学中药学一级学科开放课题项目(2011ZYX2-011)

摘　　要：为了研究辅料和温度对狼毒(月腺大戟)成分的影响,建立不同炮制品的特征图谱,该文采用不同方法和温度制备炮制品,采用Welchorm-C18色谱柱(4.6 mm×250 mm,5μm),检测波长226 nm,柱温25℃,流动相乙腈-水,梯度洗脱,测定不同炮制品中有效成分狼毒乙素、狼毒丙素及毒效成分岩大戟内酯B的含量,同时建立各自特征指纹图谱,比较其相似度。结果发现有效成分狼毒乙素、狼毒丙素的含量随着温度的升高基本呈现先上升后下降的趋势,而岩大戟内酯B的含量则随着温度的升高呈现不断降低的趋势。不同炮制品的HPLC图谱差别较大,相似度低。该研究发现辅料和温度对月腺大戟化学成分的影响较大。醋制法较其他方法更能发挥减毒保效的作用,辅料醋不能被化学试剂乙酸替代。To prepare processed products with different methods, in order to study the impact of auxiliary materials and tem- perature on chemical components of Euphorbia ebracteolata, and establish specific chromatograms of different processed products. Wel- chonn-C18 column（4.6mm×250mm,5μm） was used and eluted with a gradient program, with acetonitrile（ A） -water（ B） . The column temperature was 25 ℃, and the detection wave length was set at 226 nm. The aim was to determine the content of effective components in different processed products ebracteolata cpd B, ebracteolata cpd C and jolkinolide B and establish respective characteris- tic fingerprints to compare with similarity. The results showed that the content of ebracteolata cpd B, ebracteolata cpd C first increased and then decreased with the rise in temperature. Different processed products showed significant difference in HPLC spectrograms, with a low similarity. This study showed great impacts of auxiliary materials and temperature on chemical components of E. ebracteolata. As the vinegar processing method had higher attenuation and and synergistic effects than other methods, the auxiliary material vinegar cannot be replaced by chemical reagent acetic acid.

关 键 词：狼毒(月腺大戟) 炮制 醋制 

分 类 号：R284[医药卫生—中药学]