参莲提取物对兔颈总动脉低剪应力模型血流及血管病理形态的影响  

Effect of Shenlian extracts on blood flow and vessel pathological changes in rabbits carotid atherosclerosis model induced by low shear stress

作　　者：周淑媛[1] 王迎寒[1,2] 李玉洁[1] 杨庆[1] 巩仔鹏[1] 阮从潇[1] 阚晓溪[1] 张瑞杰[1] 朱晓新[1] 

机构地区：[1]中国中医科学院中药研究所,北京100700 [2]承德医学院中药研究所,河北承德067000

出　　处：《中国中药杂志》2013年第10期1595-1600,共6页China Journal of Chinese Materia Medica

基　　金：国家科研院所技术开发研究专项(NCSTE220072JKZX2301);国家自然科学基金面上项目(30973901);中国中医科学院基本科研业务费自主选题项目(ZZ2006117)

摘　　要：血流动力学的改变对脂质沉积和血管内皮的影响与动脉粥样硬化(artherosclerosis,As)的发生密切相关。本实验以颈总动脉套管和高脂饵食法建立家兔局部低剪切应力As模型,通过检测造模2,4,8周的血流量、切应力、血脂水平及血管病理形态,发现颈总动脉套管法在近心端可形成局部低剪应力;阿托伐他汀和参莲提取物呈时间依赖性和剂量依赖性地增加近心端血流量(P<0.05),降低血清总胆固醇(total cholesterol,TC)、甘油三酯(triglyceride,TG)、低密度脂蛋白胆固醇(lowdensity lipoprotein cholesterol,LDL-C)水平(P<0.05),减少As模型家兔近心端血管病变程度。本研究首次证实了参莲提取物(丹参和穿心莲组成)对As斑块形成的抑制作用与改善血流有关。Lipid accumulation in the vessel wall and tunica intima vasorum pathological changes are important factors in the development of atherosclerosis, which are closely related with hemodynamics. In this paper, we established a model of local low shear stress in rabbits using carotid artery cannula and a high cholesterol diet for 2 weeks, 4 weeks and 8 weeks. The effects of Shenlian extract on blood flow, vascular pathology formation and lipid metabolism were assessed by electromagnetic blood flow meter and hematoxy- lin-eosin staining of the proximal end in carotid artery at different times. The results demonstrate that the relationship between blood flow and shear stress for control, atorvastatin, Shenlian extract high-dose, Shenlian extract middle-dose, and Shenlian extract low-dose were linearly related. The blood flow and the shear stress of proximal end in carotid artery of Shenlian extract （1.12,2. 24,4. 48 g · kg^-1）, and atorvastatin （4.7 × 10^-4 g · kg^-1） were significantly （P 〈0. 05）increased compared with the control. Plasma total cholesterol （TC）, low density lipoprotein cholesterol （ LDL-C ） , and high density lipoprotein cholesterol （HDL-C） were markedly de- creased with the increasing of dose and time. This study is the first to prove that the inhibition of Shenlian extract on low shear stress （LSS） induces rabbits carotid atherosclerosis with increasing blood flow and decreasing lipids and vessel pathological changes.

关 键 词：动脉粥样硬化 参莲提取物 剪切应力 血流动力学 

分 类 号：R285.5[医药卫生—中药学]