固相萃取-高效液相色法测定饮用水中的丙烯酰胺  

Determination of Acrylamide in Drinking Water by Solid Phase Extraction Coupled with High Performance Liquid Chromatographic

作　　者：马颖 常希平 张建柱 王莹 王茹意[2] 覃素姿 王宛[2] 

机构地区：[1]国家城市供水水质监测网滨海监测站,天津300457 [2]天津博纳艾杰尔科技有限公司,天津300451

出　　处：《现代科学仪器》2013年第2期153-156,159,共5页Modern Scientific Instruments

摘　　要：本文建立了固相萃取-高效液相色法测定水中丙烯酰胺的方法。经过优化实验,最终方法确定使用Cleanert AcA固相萃取柱进行富集丙烯酰胺,同时去除水中的干扰物质;使用亲水的C18色谱柱,以水做流动相,对生活饮用水进行浓度分别为0.1mg/L、0.9mg/L的加标回收率实验,每个浓度共进行7组平行实验,相对标准偏差分别为4.8%、4.0%,回收率范围在87%～107%,最低检测限量浓度为0.1mg/L。将该方法应用于自来水厂水样的实际检测,结果表明该方法适合于水体中丙烯酰胺项目的检测。A simple and sensitive solid phase extraction（SPE） coupled to high performance liquid chromatographic（HPLC） method has been developed to quantify acrylamide in water.After a preconcentration by Cleanert AcA,analyte was chromatographed on Venusil MP C18 using water as the mobile phase.The mean recoveries of spiked samples at the levels of 0.1mg/L,0.9mg/L were in the range of 87%-107%,the relative standard deviations（n=7） were 4.8%,4.0%,respectively.The detection limit of acrylamide is 0.1mg/L.Applying this method to detect drinking water from waterworks,the results showed that the method is suitable for determining acrylamide in drinking water.

关 键 词：丙烯酰胺 饮用水 固相萃取 高效液相色谱 

分 类 号：R123.1[医药卫生—环境卫生学] O657.72[医药卫生—公共卫生与预防医学]