硒化壳聚糖对种公鸡组织硒含量、硒酶活性及其基因表达的影响  

Effects of Seleno-Chitosan on Tissue Selenium Content,Activities and Gene Expressions of Selenoenzymes in Breeder Cocks

作　　者：赵晓旭[1] 杨佐君[1] 滑静[2] 王晓霞 张洁[4] 

机构地区：[1]北京农学院动物科学技术学院,北京102206 [2]北京农学院生物科学与工程学院,北京102206 [3]北京市昌平区区委,北京102200 [4]北京农业职业技术学院畜牧兽医系,北京102442

出　　处：《动物营养学报》2013年第5期1085-1092,共8页CHINESE JOURNAL OF ANIMAL NUTRITION

基　　金：2012年现代农业产业技术体系北京市家禽创新团队营养与饲料功能研究室岗位专家(PXM2012_014207_000166)

摘　　要：本试验旨在比较研究硒化壳聚糖(SC)和亚硒酸钠(SS)对海兰褐种公鸡组织硒沉积量,硫氧还蛋白还原酶(TrxR)、Ⅰ型脱碘酶(IDⅠ)活性及TrxR2、IDⅠ基因表达的影响。选取280只20周龄海兰褐种公鸡,随机分为7个处理,每个处理4个重复,每个重复10只鸡。将SC和SS分别以0.4、0.8和1.2 mg/kg 3个硒水平添加到海兰褐种公鸡基础饲粮中,对照组饲喂基础饲粮,进行为期35 d饲养试验。结果表明:1)不同的硒源和硒添加水平对种公鸡生长性能影响不显著(P>0.05)。2)在睾丸中,1.2 mg/kg SS添加组硒含量显著高于对照组(P<0.05),1.2 mg/kg SC添加组硒含量极显著高于对照组(P<0.01);在肾脏中,0.8 mg/kg SS、1.2 mg/kg SS以及1.2 mg/kg SC添加组硒含量均显著高于对照组(P<0.05)。3)饲粮中添加0.4 mg/kg SS极显著提高了种公鸡睾丸TrxR活性(P<0.01),添加0.4 mg/kg SC显著了提高种公鸡睾丸TrxR活性(P<0.05),但只有添加0.4 mg/kg SC才能极显著提高肾脏IDⅠ活性(P<0.01)。4)0.4 mg/kg SC添加组睾丸TrxR2基因mRNA相对表达水平最高,但各组均差异不显著(P>0.05);随着硒添加水平的升高,在SS添加组,睾丸TrxR2基因mRNA相对表达水平呈现先升高后下降的趋势,而在SC添加组,睾丸TrxR2基因mRNA相对表达水平呈现先升高后下降再升高的趋势。与对照组比较,0.4 mg/kg SS添加组肾脏IDⅠ基因mRNA相对表达水平显著降低(P<0.05)。由此可知,SC对海兰褐种公鸡组织硒沉积,TrxR、IDⅠ活性以及TrxR2、IDⅠ基因表达有较大影响;建议添加低水平(0.4 mg/kg)SC。This experiment was conducted to compare the effects of seleno-chitosan （SC） and sodium selenite （SS） on tissue selenium retention, thioredoxin reductase （TrxR） and iodothyronine deiodinase I （ID I ） ac- tivities and mRNA relative expression levels of TrxR2 and ID I genes in Hy-Line breeder cocks. Two hundred and eighty 20-week-old Hy-Line breeder cocks were allocated randomly into seven treatments with four repli- cates per treatment and ten breeder cocks per replicate. Control group was fed a basal diet, while the six other groups were fed the basal diet supplemented with 0.4, 0.8 and 1.2 mg/kg selenium from SC or SS, respec- tively. The experiment lasted for 35 days. The results showed as follows ： 1 ） Se source and supplemental level did not influence the growth performance of breeder cocks （ P 〉 0.05 ）. 2 ） The selenium content in testis of breeder cocks of 1.2 mg/kg SS supplemental group and 1.2 mg/kg SC supplemental group was significantly higher than that of control group （P 〈0.05 and P 〈0.01, respectively）. The selenium content in kidney of breeder cocks of 0.8 mg/kg SS supplemental group, 1.2 mg/kg SS supplemental group and 1.2 mg/kg SC supplemental group had a significant increase compared with control group （ P 〈 0.05 ）. 3 ） TrxR activity in testis of breeder cocks of 0.4 mg/kg SS supplemental group and 0.4 mg/kg SC supplemental group was sig- nificantly higher than that of control group （ P 〈 0.01 and P 〈 0.05, respectively）, but only ID I activity in kidney of 0.4 mg/kg SC supplemental group was significantly higher than that of control group （ P 〈 0.01 ）. 4 ） The mRNA relative expression level of TrxR2 gene in testis of breeder cocks of 0.4 mg/kg SC supplemen- tal group was the highest of all groups, but there was no significant difference among all groups （ P 〉 0.05 ）. With the increase of supplemental selenium level, the mRNA relative expression level of TrxR2 gene in testis was increased at first, and then descended in SS suppleme

关 键 词：硒化壳聚糖 组织硒含量 硫氧还蛋白还原酶 Ⅰ型脱碘酶 基因表达 

分 类 号：S816.7[农业科学—饲料科学]