A—FABP与妊娠期高血压疾病的相关性研究  

Correlation study on A-FABP and hypertensive disorder complicating pregnancy

作　　者：李亚萍[1,2] 裴美丽[3] 王明旭[1] 

机构地区：[1]西安交通大学医学院,陕西西安710061 [2]西安市中心医院,陕西西安710003 [3]西安交通大学医学院第一附属医院,西安717061

出　　处：《中国妇幼健康研究》2013年第2期215-218,共4页Chinese Journal of Woman and Child Health Research

基　　金：陕西省科学技术研究发展计划资助项目（2010K01-161）

摘　　要：目的研究妊娠期高血压疾病患者胎盘组织中脂肪细胞型脂肪酸结合蛋白（A—FABP）水平的变化及临床意义。方法采用酶联免疫吸附双抗体夹心法及免疫组化方法检测72例妊娠期高血压疾病患者（A组：妊娠期高血压24例；B组：轻度子痫前期24例；c组：重度子痫前期24例）及24例正常妊娠妇女（对照组）血清及胎盘组织中A—FABP水平。结果妊娠期高血压疾病组血清A—FABP水平明显高于对照组（33．854-8．42ng／mLVS14．35±5．54ng／mL；t=12．961，P〈0．01）。A、B、C各组血清中A—FABP水平均较正常对照组高，并随疾病进展逐渐升高（依次为17．42±8．21ng／mL，32．38±11．21ng／mL，35．55±12．50ng／mL；t值分别为1．523、7．064、9．224，均P〈0．05）；妊娠期高血压疾病组胎盘组织中A—FABP表达较对照组明显升高（Z=-3．485，P〈0．01），且随病情进展表达增强（，=0．552，P〈0．001）。妊娠期高血压疾病组和对照组两组血清中A—FABP水平与胎盘组织A—FABP表达均呈正相关（r=0．501，P〈0．05；r=0．528，P〈0．05）。结论A—FABP的表达可能参与了妊娠期高血压疾病的发生、发展。Objective To investigate the expression of adipocyte fatty acid-binding protein（A-FABP） in maternal serum and placenta of patients with hypertensive disorder complicating pregnancy （HDCP） , and its clinical significance. Methods Enzyme linked immunosorbent assay （ELISA） and immunohistochemistry were used to detect the expression of A-FABP in serum and placenta of 72 patients with HDCP （ Group A ： 24 cases of gestational hypertension ; Group B ： 24 cases of mild preeclampsia ; Group C ： 24 cases of severe preeclampsia） and 24 normal pregnant women （control group）. Results Serum level of A-FABP in HDCP groups （33.85 ± 8.42ng/mL） was significantly higher than that in control group （14.35 ±5.54ng/mL, t = 12. 961 ,P 〈0.01 ）. Serum level of A-FABP in group A, B, or C （17.42 ± 8.21ng/mL, 32.38 ± 11.21ng/mL, 35.55 ±~ 12.50ng/mL） was significantly higher than that in control group, and the level gradually elevated according to the progression of disease （ t value was 1,523, 7. 064 and 9. 224, respectively, all P 〈 0.05 ）. The expression of A- FABP in the placenta of HDCP groups was significantly higher than that in control group （Z = -3. 485,P 〈0.01 ）, and that elevated gradually with the progression of disease （r = 0.552, P 〈 0. 001 ）. Both in HDCP groups and control group, levels of A-FABP in maternal serum was positively related with those in placenta （ r = 0. 501, P 〈 0.05 ; r = 0. 528, P 〈 0.05 ）. Conclusion The expression of A-FABP might play a role in the pathogenesis and development of HDCP.

关 键 词：脂肪细胞型脂肪酸结合蛋白 妊娠期高血压疾病 血清 胎盘组织 

分 类 号：R14[医药卫生—公共卫生与预防医学]