葡萄籽原花青素对小鼠睾丸扭转复位后生精功能的保护作用  

Protective effect of grape seed proanthocyanidin on spermatogenesis following testicular torsion /detorsion in mice

作　　者：尚瑞[1] 郑新民[1] 夏志平[1] 张琳[1] 郑雪皎[2] 

机构地区：[1]武汉大学中南医院泌尿外科,湖北武汉430071 [2]湖北医药学院附属人民医院药学部,湖北十堰442000

出　　处：《中华男科学杂志》2013年第5期409-413,共5页National Journal of Andrology

摘　　要：目的:探讨葡萄籽原花青素(GSP)对小鼠睾丸扭转复位后生精功能的保护作用。方法:24只健康雄性昆明小白鼠(8周龄,25～27 g)随机分为3组:对照组、扭转组、治疗组,每组8只。扭转组及治疗组建立单侧睾丸扭转复位动物模型,治疗组于扭转复位前30 min腹腔注射GSP(50 mg/kg),术后采用腹腔注射方式连续给药3 d,每天1次,每次50 mg/kg。扭转组方法同治疗组,治疗同体积生理盐水。术后第4天取扭转侧睾丸,检测组织病理学参数和生精细胞凋亡指数(AI),并检测睾丸组织超氧化物歧化酶(SOD)和丙二醛(MDA)含量。对照组行假手术。结果:治疗组与扭转组相比,Johnsen评分上升[(7.38±0.92)分vs(5.00±1.85)分,P<0.05],生精小管直径略增大[(178.75±1.58)μm vs(176.50±1.60)μm,P>0.05],生精细胞层数增加[(5.75±0.71)层vs(3.75±1.03)层,P<0.05],生精细胞凋亡指数AI明显降低[(16.25±1.67)%vs(40.50±1.60)%,P<0.05)],SOD活性明显上升[(52.67±3.57)U/mg prot vs(29.04±4.46)U/mg prot,P<0.05],MDA含量明显下降[(2.91±0.04)nmol/mg prot vs(4.63±0.05)nmol/mg prot,P<0.05]。结论:GSP对小鼠睾丸扭转复位后生精功能损伤有明显的保护作用,其作用机制可能与其能清除氧自由基、抑制脂质过氧化、提高机体抗氧化能力有关。Objective ： To investigate the protective effect of grape seed proanthocyanidin （GSP） on spermatogenesis following testieular torsion/detorsion in mice. Methods： Twenty-four healthy male Kunming mice, aged 8 weeks and weighing 25 - 27 g, were randomly divided into a control, a torsion and a treatment group, each containing 8 animals. The unilateral testicular torsion / detorsion model was established in the treatment and torsion groups. Thirty minutes before detorsion, the animals of the treatment group were injected intraperitoneally with 50 mg/kg GSP, and those of the torsion group with normal saline at the same dose, both for 3 days postoperatively. On the 4th day after surgery, ipsilateral orehieetomies were performed to detect histopathological changes, the levels of superoxide dismutase （SOD） and malondialdehyde （MDA）, and the apoptotic index （AI） of germ cells in all the mice. Results： Compared with the torsion group, the treated mice showed significantly increased Johnsen score （5.00 ± 1. 85 vs 7.38 ± 0.92, P 〈 0.05）, seminiferous tubule diameter （[176.50 ＋ 1.60] μm vs [ 178.75 ＋ 1.58] μm, P 〉0.05）, spermatogenic cell layers （3.75 ± 1.03 vs 5.75 ± 0.71, P 〈 0.05 ） and SOD activity （ [ 29.04 ± 4.46 ] U/mg prot vs [ 52.67 ± 3.57 ] U/mg prot, P 〈 0.05）, but remarkably reduced level of MDA （ [4.63 ±0.05] nmol/mg prot vs [2.91 ±0.04] nmol/mg prot, P〈0.05） and AI of germ cells （ [40.50 ＋1.601% vs [16.25 ＋1.67] %, P〈0.05）. Conclusion： Grape seed proanthocyanidin has a protective effect against spermatogenic injury in mice, the mechanisms of which may be related to its actions of scavenging oxygen free radicals, inhibi- ting lipid peroxidation and improving the antioxidant ability of the body.

关 键 词：葡萄籽原花青素 睾丸扭转 复位 超氧化物歧化酶 丙二醛 细胞凋亡 小鼠 

分 类 号：R285.5[医药卫生—中药学]