人精母细胞减数分裂重组异常对非梗阻性无精子症患者病理结果影响的初步研究  

Influence of recombination abnormality in spermatocyte meiosis on the pathology of patients with non-obstructive azoospermia

作　　者：赵连明[1] 姜辉[1] 洪锴[1] 陆敏[2] 唐文豪[1] 毛加明[1] 杨庆岭[3] 马潞林[1] 

机构地区：[1]北京大学第三医院泌尿外科,北京100191 [2]北京大学基础医学院病理学系,北京100191 [3]合肥微尺度物质科学国家实验室,中国科学技术大学生命科学学院分子细胞遗传学实验室,安徽合肥230027

出　　处：《中华男科学杂志》2013年第5期425-428,共4页National Journal of Andrology

摘　　要：目的:初步探讨人精母细胞减数分裂重组异常对非梗阻性无精子症患者病理结果的影响。方法:6例非梗阻性无精子症患者,经过睾丸穿刺活检取得睾丸组织。每例患者的睾丸组织1式2份,1份用于病理检查,另1份用于免疫荧光染色,观察联会复合体的情况,同源染色体上重组位点的数目。寻找重组异常与病理结果之间的关系。结果:6例非梗阻性无精子症患者的病理结果显示:3例患者生精小管基底膜增厚,3例患者生精小管基底膜萎缩。生精小管萎缩的3例患者每个细胞常染色体上存在MLH1,数量范围是30～50个,而生精小管基底膜增厚的3例患者每个细胞常染色体上存在MLH1,数量范围是10～50个。结论:同源染色体重组频率波动范围的增大可能是导致患者生精小管基底膜增厚乃至管腔闭塞的原因之一。Objective： To primarily study the influence of recombination abnormality in human spermatocyte meiosis on the pathology of the patients with non-obstructive azoospermia （NOA）. Methods： We obtained testis tissues from 6 NOA patients by testicular biopsy and divided the tissue of each patient into 2 portions, one for pathological examination and the other for immunofluorescent staining. We observed the synaptonemal complex and the numbers of the recombination sites on homologous chromosomes, and analyzed the relationship between abnormal recombination and pabtological findings. Results： Pathological examination showed that the basement membrane of the seminiferous tubules was thickened in 3 of the cases and atrophied in the other 3, the number of autosoreal MLH1 foci in a spermatoeyte ranging from 10 to 50 in the former 3, and from 30 to 50 in the latter 3. Conclusion ： The increased range of the homologous ehoromosomal recombination frequency may be one of the possible factors for the thickening of seminiferous tubule basement membrane and even lumen occlusion in NOA patients.

关 键 词：无精子症 病理 重组 减数分裂 

分 类 号：R698.2[医药卫生—泌尿科学]