OCTN2在1例正常生育男性附睾中的表达报告  

Expression of OCTN2in human epididymis and its significance

作　　者：李东[1] 冯自卫[1] 毕学成[1] 冼志勇[1] 叶楚津[1] 罗耀雄[1] 王怀鹏[1] 郑祥光[1] 刘久敏[1] 

机构地区：[1]广东省人民医院泌尿外科,广州510080

出　　处：《临床泌尿外科杂志》2013年第5期383-385,共3页Journal of Clinical Urology

基　　金：广东省科技计划基金资助项目(编号2010B080701090)

摘　　要：目的:研究人类附睾有机阳离子转运子2(OCTN2)的mRNA表达特征及蛋白表达特征,为进一步探讨附睾肉碱转运机制提供理论依据。方法:采用RT-PCR方法检测人类附睾组织头部、体部及尾部OCTN2基因的表达;并用Westernblot方法检测该基因在附睾中的蛋白表达,计算其在蛋白水平的相对表达量。结果:OC-TN2mRNA在人附睾头、体、尾组织中均有OCTN2表达,表现为附睾头部表达较弱,而附睾体、尾部分表达丰富;OCTN2蛋白在人附睾头部表达较弱,表达量为(0.71±0.09),附睾体、尾部分表达较丰富,表达量分别为(0.95±0.22)与(0.99±0.15)。结论:两种方法均证实有机阳离子转运子2在人类附睾中有表达,且呈现附睾头部表达较弱,附睾体、尾部分表达丰富的特征,为进一步研究附睾肉碱转运机制奠定基础。To study the expression of OCTN2 mRNA and protein in human epididymis so as to provide a further theoretical basis for the mechanism of I. Carnitine transport. Method：We collected epididymides from a prostate cancer patient and determined the expressions of OCTN2 mRNA and it＇s protein in the caput, corpus and cauda of the epididymis by RT-PCR and Westernblot and calculated the relative content of the protein. Result：OCTN2 Mrna was expressed in the caput,corpus and cauda of the epididymis and the expression level in the corpus and cauda of the epididymis were higher than in the caput . OCTN2 protein was also expressed in human epididy mis and the relative content was （0.71±0.09） in the caput of the epididymis. It was lower than the relative content （0.95±0.22） and （0.99±0. 15） respectively in the corpus and cauda of the epididymis. Conclusion：The ex pression of OCTN2 mRNA and protein in human epididyrnis was certificated by RT PCR and westernblot. They both indicated that the expression level was low in the caput of the epididymis and the expression level was high in the corpus and cauda of the epididymis. This conclusion will play an important role in the study of the mechanism of L Carnitine transport.

关 键 词：人类附睾 OCTN2 蛋白表达 WESTERNBLOT 

分 类 号：R69[医药卫生—泌尿科学]