巨细胞病毒感染后雄鼠生殖细胞凋亡与睾丸NF-κB p65的表达研究  

Apoptosis of Reproductive Cells and Expression of NF-κB p65 in Testes of Male Mice with MCMV Infection

作　　者：熊锦文[1] 胡廉[2] 官黄涛[2] 赵玉梅[1] 徐红[1] 于建萍[1] 熊承良[2] 

机构地区：[1]武警湖北总队医院妇科,武汉市430061 [2]华中科技大学同济医学院计划生育研究所,武汉市430030

出　　处：《医学分子生物学杂志》2013年第1期58-62,共5页Journal of Medical Molecular Biology

摘　　要：目的研究鼠巨细胞病毒（MCMV）感染对雄鼠睾丸生殖细胞凋亡的影响，探讨睾丸组织核因子-κB亚基p65亲和肽（NF-κBp65）表达与生殖细胞凋亡的相关性。方法建立小鼠睾丸MCMV感染模型，设立实验组与对照组，分别于感染不同时段（小鼠一个生精周期内），采用Hoechst33258染色法对小鼠睾丸组织生精小管及间质细胞进行凋亡检测，采用免疫组织化学sP法检测小鼠睾丸组织内NF-κB065的表达。结果睾丸间质细胞的凋亡率在MCMV感染的第1天开始增多（P〈0．05），第4-6天达到高峰。第21天、第38天恢复正常。生精小管管周肌样上皮细胞的凋亡数在感染的第1天开始增多（P〈0．05）．第6—9天达到高峰（P〈0．001），第21天、第38天也恢复正常。生精细胞的凋亡数在感染的第2天显著增加（P〈0．05），第6天达到高峰（P〈0．001）；第14天起逐渐恢复正常。睾丸组织中NF-κBp65表达在MCMV感染第2天起开始增加（P〈0．05），感染的第6—9天增至高峰（P〈0．001），随感染时间延长逐渐下降，第21～28天恢复正常。结论MCMV急性感染可诱导睾丸生殖细胞凋亡，其凋亡程度与睾丸组织NF-κBp65表达呈正相关。提示NF-κBp65参与并调控了MCMV感染所致的生殖细胞凋亡，这可能是MC-MV感染的致病机制及机体抵御MCMV感染机制之一。Objective To study the effect of MCMV infection on the apoptosis of reproductive cells in male mice and the relationship between NF-κB p65 expression in testes and reproductive cell apoptosis after MCMV infection. Methods Murine testicular MCMV infection models were estab- lished and experiment and control groups designed in terms of different periods of MCMV infection （within one seminiferous cycle） . The apoptosis of seminiferous tubule and interstitial cells in mouse testicular tissues was detected by Hoechst33258 staining. The immunohistochemical SP method was used to determine the expression level of NF-κB p65 in testicular tissue. Results The apoptosis rate of interstitial cells began to increase on the first day of MCMV infection （ D1 ） （ P 〈 0.05 ）, reached a peak on D4 to D6 （P 〈 0. 001 ） , and returned to normal on D21 and D38. The number of apoptot- ic peritubular myoid ceils in the convoluted seminiferous tubules was increased on D1 （P 〈0. 05）, reached the peak on D6 to D9 （P 〈 0. 001 ）, and also returned to normal on D21 and D38. The number of apoptotic seminiferous cells were significantly enhanced on D2 （ P 〈 0. 05 ）, peaked on D6 （ P 〈 0. 001 ）, and returned to normal on D14. The expression of NF-κB p65 protein in testiculartissues was increased since D2 （ P 〈 0. 05） , and reached a peak on D6 to D9 （P 〈 0. 001 ） . As in- fection continued, the expression of NF-κB p65 was decreased and returned to normal on D2I and D38 （P 〉0.05） . Conclusion Acute MCMV infection could induce the apoptosis of reproductive cells of the male mice. There was a positive correlation between the apoptotic degree and the NF-κB p65 expression. It was suggested that NF-κB p65 is implicated in the reproductive cell apoptosis after MCMV infection, which may be a pathogenic mechanism of MCMV infection and a mechanism for self-defense to MCMV infection.

关 键 词：鼠巨细胞病毒 睾丸 生殖细胞 凋亡 NF-ΚB P65 

分 类 号：R697.22[医药卫生—泌尿科学] R373.9[医药卫生—外科学]