补阳还五汤对腹主动脉缩窄致大鼠心肌肥厚肌浆网钙摄取功能的影响  

Effects of Buyang Huanwu Decoction on the Sarcoplasmic Reticulum Calcium Uptake in Abdominal Aortic Constriction Induced Myocardial Hypertrophic Rats

作　　者：顾燕频[1,2] 廖月玲[1] 章忱[1] 郭炜[1] 卫洪昌[1] 吕嵘[1] 

机构地区：[1]上海中医药大学病理教研室,上海201203 [2]浙江中医药大学附属第三医院老年科,杭州310000

出　　处：《中国中西医结合杂志》2013年第5期627-631,共5页Chinese Journal of Integrated Traditional and Western Medicine

基　　金：国家自然科学基金资助项目(No.30973821);上海市教育委员会重点学科建设资助项目(No.J50301);上海市教育委员会科研创新资助项目(No.10YZ52)

摘　　要：目的观察补阳还五汤对腹主动脉缩窄致大鼠心肌肥厚的影响以阐明其对心肌肌浆网钙泵(sarcoplasmic reticulum calcium,SERCA2a)摄取的分子调控机制。方法采用腹主动脉缩窄法制作压力超负荷大鼠心肌肥厚模型。造模4周后,分为假手术组(S组),模型组(M组),依那普利组(E组),补阳还五汤组(BYHWD组),给药12周后观察各组大鼠的左室收缩压(left ventricular systolic pressure,LVSP)、左室舒张末压(left ventricular end-diastolic pressure,LVEDP)、左室内压最大上升速度(+dp/dtmax)、左室内压最大下降速度(-dp/dtmax)、心输出量(cardiac output,CO)、心脏及左心质量指数变化(heart mass index,HMI;left ventricular mass index,LVMI),并且采用ELISA法检测血清中心房利钠肽(atrial natriuretic peptide,ANP)、脑钠肽(brain natriuretic peptide,BNP)的含量,以及观察各组肌浆网中SERCA2a活性以及SERCA2a、受磷蛋白(phospholamban,PLN)及磷酸化的表达情况。结果与S组比较,M组LVSP及LV-EDP显著升高(P<0.05),-dp/dtmax及CO下降明显(P<0.05),心肌组织明显肥厚,血清中ANP、BNP含量增高(P<0.05),SERCA2a活性及表达量下降(P<0.05),SERCA2a/PLN比值及PLN磷酸化程度降低(P<0.05)。与M组比较,BYHWD组可明显降低LVEDP,提高-dp/dtmax及CO(P<0.05);可显著减轻心肌肥厚,降低血清ANP及BNP含量(P<0.05),增加SERCA2a活性(P<0.05),提高SERCA2a、Ser16及Thr17蛋白表达相对含量(P<0.05);具有显著提高SERCA2a/PLN比值和PLN磷酸化程度的作用(P<0.05)。结论补阳还五汤能够改善心衰大鼠血流动力学,提高心输出量,减轻心肌肥厚及提高心肌SER-CA2a摄取功能。Objective To investigate effects of Buyang Huanwu Decoction （BYHWD） on the rats′ myocardial hypertrophic model induced by abdominal aortic constriction, and to clarify the molecular regulatory mechanisms for sarcoplasmic reticulum calcium uptake. Methods Hypertrophic myocardium rat model was induced by abdominal aorta constriction （AAC）. Four weeks after modeling, rats were randomly divided into the sham-operation group （Group S）, the AAC model group （Group M）, the Enalapril group （Group E）, and the BYHWD treatment group （Group BYHWD）, respectively. The left ventricular systolic pressure （LVSP）, left ventricular end-diastolic pressure （LVEDP）,＋dp/dtmax,-dp/dtmax, cardiac output （CO）, heart mass index （HMI）, and left ventricular mass index （LVMI） were observed in each group after 12-week medication. The serum contents of the atrial natriuretic peptide （ANP） and brain natriuretic peptide （BNP） were detected using ELISA. The SERCA2a activity, the expressions of SERCA2a, phospholamban （PLN）, and PLN phosphorylation were observed finally. ResultsCompared with Group S, LVSP and LVEDP significantly increased,-dp/dtmax and CO obviously decreased, the myocardial tissue was obviously thickened, the serum contents of ANP and BNP increased, the activity and expression of SERCA2a decreased, the SERCA2a/PLN ratio and PLN phosphorylation degree decreased in Group M （all P 0.05）. Compared with Group M, LVEDP obviously decreased,-dp/dtmax and CO obviously increased, the hypertrophy myocardial tissue was obviously lessened, the serum contents of ANP and BNP decreased, the activity of SERCA2a increased, the relative expression contents of SERCA2a, Ser16, and Thr17 were elevated in Group BYHWD （all P 0.05）. BYHWD had significant roles in elevating the SERCA2a/PLN ratio and PLN phosphorylation degree （P 0.05）. Conclusion BYHWD could significantly improve hemodynamics of heart failure rats, elevate CO, lessen cardiac hypertrophy, and improve the capabilities for s

关 键 词：补阳还五汤 心肌肥厚 钙摄取 肌浆网钙泵 受磷蛋白 

分 类 号：R285.5[医药卫生—中药学]