采用均匀设计研究南沙参、北沙参配伍藜芦相反的毒性作用规律  

Research on the Incompatibility of Radix Adenophorae , Radix Glehniae Combined with Veratrum nigrum L. by Uniform Designed Toxicity Assay

作　　者：朱冠秀[1,2] 王宇光[2] 李飞[1,2] 杨亮[2] 陆倍倍[2] 马增春[2] 梁乾德[2] 肖成荣[2] 谭洪玲[2] 汤响林[2] 张伯礼[3] 高月[1,2] 

机构地区：[1]中南大学药学院,长沙410013 [2]军事医学科学院放射与辐射医学研究所,北京100850 [3]中国中医科学院,北京100700

出　　处：《中国中西医结合杂志》2013年第5期686-690,共5页Chinese Journal of Integrated Traditional and Western Medicine

基　　金：国家重点基础研究发展计划项目(No.2011CB505304;2012CB518402);北京市自然科学基金资助项目(No.7112110);国家自然科学基金资助项目(No.81073149)

摘　　要：目的旨在为南沙参、北沙参与藜芦不同比例配伍是否产生相反作用提供动物急性毒性数据,并考察煎煮因素是否与毒性相关。方法采用均匀设计方法,将南沙参、北沙参分别与藜芦进行2因素7水平配比优化,以急性毒性实验小鼠死亡率作为毒性指标,考察不同配比情况对藜芦毒性的影响;并利用南沙参、北沙参配伍藜芦合煎液和合并液分别对毒性进行考察,以期探寻配伍毒性是否与共同煎煮因素有关。结果南沙参、北沙参与藜芦合用后,毒性作用主要由藜芦来介导且毒性随藜芦剂量的增加而增大。南沙参、北沙参与藜芦在1∶1配伍时合煎液毒性大于合并液,提示南沙参、北沙参与藜芦共煎过程可能对藜芦中毒性成分的溶出产生了影响,进而表现为合煎液毒性大于合并液毒性。结论南、北沙参与藜芦配伍在动物水平上表现出相反的配伍规律,提示临床应用此类药物应谨慎。Objective To study the toxicity changes of different proportions of Radix Adenophorae , Radix Glehniae combined with Veratrum nigrum L., thus providing acute toxicity data and investigating whether decoction factors were correlated with toxicity. Methods The uniform design method was used by two factors and seven levels to investigate the toxicity changes in different proportions of Radix Adenophorae , Radix Glehniae combined with Veratrum nigrum L. The decoction factors were also investigated. Results The compatibility toxicity was affected mainly by Veratrum nigrum L. and the toxicity increased along with increased doses of Veratrum nigrum L. The toxicity of co-decoction was higher than mixed decoction in the same dosage of Radix Glehniae and Veratrum nigrum L. The promotion of the dissolution of the toxic component of Veratrum nigrum L. in co-decoction may be the cause of the higher toxicity. Conclusion Radix Adenophorae and Radix Glehniae combined with Veratrum nigrum L. resulted in higher toxicity, which indicated that the incompatibility between Radix Adenophorae , Radix Glehniae , and Veratrum nigrum L. In clinic practice, a prescription contained these drugs should be avoided.

关 键 词：十八反 均匀设计 急性毒性 南沙参 北沙参 藜芦 

分 类 号：R285.1[医药卫生—中药学]