CaMKⅡα在FMR1基因敲除小鼠脑组织中的表达  

Expression of calcium/calAulin kinaseⅡα in fragile X mental retardation 1 gene knockout brain tissues in mice

作　　者：韦朝霞[1,2,3] 

机构地区：[1]广州医学院荔湾医院神经内科,广州,510170 [2]广州医学院第二附属医院神经病学研究所,广州,510260 [3]广州医学院动物实验中心,广州,510182

出　　处：《中华神经医学杂志》2013年第5期444-447,共4页Chinese Journal of Neuromedicine

基　　金：广东省卫生厅基金

摘　　要：目的观察FMR，基因敲除型（KO）小鼠脑组织中钙／钙调素依赖蛋白激酶Ⅱα（CaMKⅡα）表达的改变，探讨CaMKⅡα是否为脆性X综合征相关蛋白（FMRP）的下调蛋白。方法PCR鉴定FVB近交系小鼠的基因型，按基因型的不同分为KO组和野生型（WT）组，每组10只。免疫组化染色检测K0及WT小鼠脑组织CaMKⅡα的表达与分布，用图像分析仪分别采集不同脑区免疫信号的吸光度（A）值进行比较。结果免疫组化染色检测显示KO与WT小鼠各个脑区普遍存存阳性信号；神经元胞浆尤其是靠近胞体的近端突起上信号呈强阳性,树突中亦有阳性信号，轴突上信号较弱；KO小鼠各脑区CaMKⅡα阳性信号的A值均较WT小鼠显著增高，差异有统计学意义（P〈0.05）。结论CaMKⅡα在成年KO小鼠各脑区的表达均显著增多，提示FMRP负性调节CaMKⅡα的表达。Objective To observe the expression of calcium/calAulin kinaseⅡα （CaMKⅡα） in the brain tissues of fragile X mental retardation 1 （FMRI） gene knockout （KO） mice to investigate whether CaMKⅡα is regulated by fragi/e X mental retardation protein （FMRP）. Methods According to the gene types of the FVB inbred mice identified by PCR, 20 mice were divided into KO group and WT （wide type） group （n= 10）. The subcellular distribution and expression of CaMKⅡα were observed by immunohistochemical staining; the mean optical density （A） values of immunostaining signal of CaMKⅡα in various brain regions, including the motor cortex, temporal cortex, amygdala, hypothalamus and hippocampus, were determined by IBAS 2.0 image-analyzed system. Results CaMKⅡα immunoreactive cells were abundantly found in all brain subregions of KO and WT mice; especial positive signal was noted in the proximal processes of neurons, so as to those in the dendrite; week signcal was observed in the axon. No distributional difference was found between KO and WT mice. As compared with those in the WT mice, the A values were distinctly increased in each brain region of KO mice with significant differences （P〈0.05）. Conclusion The increased expression of CaMKⅡα in the brain tissues of FMR 1 knockout mice hints that CaMKⅡα participates in the course of the fragile X syndrome, and FMRP may negatively regulate the expression of CaMKⅡα in physiological condition.

关 键 词：FMR-1 基因 脆性X综合征相关蛋白 钙 钙调素依赖蛋白激酶Ⅱα 

分 类 号：R285.5[医药卫生—中药学]