银丹心脑通软胶囊对血管性痴呆大鼠学习记忆能力及皮层反应性氮中介物含量的影响  

Effect of Yindan Xinnaotong capsules on reactive nitrogen intermediates levels in the cerebral cortex and learning and memory abilities of rats with cerebral ischemia

作　　者：王英 王敏 王桂华[2] 闫志慧 周艳玲 梁辉 

机构地区：[1]烟台山医院神经内科,烟台264000 [2]滨州医学院烟台校区生理教研室,烟台264003

出　　处：《中华神经医学杂志》2013年第5期466-469,共4页Chinese Journal of Neuromedicine

摘　　要：目的探讨银丹心脑通软胶囊对血管性痴呆（VD）大鼠学习记忆能力及大脑皮层反应性氮中介物含量的影响。方法将SD大鼠按随机数字表法分为假手术组、VD组、银丹心脑通软胶囊治疗组，后两组采用永久性结扎双侧颈总动脉方法制备VD模型。银丹心脑通软胶囊治疗组于手术后即刻给予银丹心脑通软胶囊60mg／（kg·d）灌胃，连续给药4周。4周后采用Morris水迷宫试验观察各组大鼠学习记忆能力的改变，采用比色法检测大鼠大脑皮层一氧化氮倒0）、诱导型一氧化氮合酶（iNOS）含量的变化。结果VD组与银丹心脑通软胶囊治疗组、假手术组比较，逃避潜伏期明显延长，原平台所在象限的搜索时间明显缩短，差异有统计学意义（P〈0．05）。银丹心脑通软胶囊治疗组较VD组逃避潜伏期明显缩短、原平台所在象限的搜索时间明显延长。差异有统计学意义（P〈0．05）。银丹心脑通软胶囊治疗组与VD组相比，大脑皮层NO和iNOS含量明显下降，差异有统计学意义似（P〈0．05）。结论银丹心脑通软胶囊能降低VD大鼠皮层NO、iNOS含量。对学习记忆能力有明显的提高作用。Objective To explore the effect of Yindan Xinnaotong capsules （YDXNT） on the reactive nitrogen intermediates in the cerebral cortex and learning and memory abilities of rats with cerebral ischemial Methods Thirty rats were randomly divided into sham-operated group, vehicle group and Yindan Xinnaotong capsule treatment group. The animal models of cerebral ischemia in the later two groups were established by two vessel occlusion. Yindan Xinnaotong capsule 60 mg/（kg.d） via intmgastric administration was given to the Yindan Xinnaotong capsule treatment group for a consecutive four weeks. The learning and memory ability changes were tested with Morris water maze and the contents of nitric oxide （NO） and inducible nitric-oxide synthase （iNOS） in the cerebral cortex were measured by colorimetric method. Results As compared with the vehicle group, the Yindan Xinnaotong capsule treatment group and sham-operated group had significantly shorter escape latency and longer searching time in the quadrant of primary platform （P〈0.05）; As compared with the vehicle group, the Yindan Xinnaotong capsule treatment group had significant decreased contents of NO and iNOS in the cerebral cortex （P〈0.05）. Conclusion Yindan Xinnaotong capsules can improve the learning and memory abilities of rats with cerebral ischemia by lowering the activity of NO and iNOS in the cerebral cortex.

关 键 词：血管性痴呆 银丹心脑通软胶囊 学习记忆能力 一氧化氮 诱导型一氧化 氮合酶 

分 类 号：R285.5[医药卫生—中药学]