姜黄素对肠易激综合征模型大鼠5-羟色胺和脑源性神经营养因子的影响  

Effect of curcumin on 5-hydroxytryptamine and brain-derived neurotrophic factor in irritable bowel syndrome rats

作　　者：余颖聪[1] 李建新[2] 吴淑娟[2] 郑亮[1] 徐英[3] 潘建春[2] 

机构地区：[1]温州市人民医院,温州医学院温州市第三临床学院,325000 [2]温州医学院药学院,温州325035 [3]美国西弗吉尼亚大学行为医学和精神病学系,26506

出　　处：《中华神经医学杂志》2013年第5期480-485,共6页Chinese Journal of Neuromedicine

基　　金：浙江省自然科学基金（Y2110896）

摘　　要：目的从脑肠轴角度明确肠易激综合征（ms）发病机制，观察姜黄素对IBS的疗效。方法48只SD大鼠按随机数字表法分为6组：正常组，IBS模型组，姜低组、姜中组、姜高组f造模前分别灌胃给予姜黄素10、20、40mg／kg），丙咪嗪组（造模前腹腔注射丙咪嗪10mg／kg），每组各8只。采用大鼠慢急性联合应激模型模拟IBS。糖水消耗试验和内脏敏感性实验检测各组大鼠抑郁样行为和肠道敏感性，高效液相法及Westernblotting分别测定大鼠大脑皮层和回肠5-羟色胺（5-HT）及脑源性神经营养因子（BDNF）的表达。结果IBS模型组大鼠糖水消耗量明显减少。肠道内脏敏感性明显升高，大脑额叶皮层5．HT、BDNF表达水平降低，回肠黏膜5-HT、BDNF表达水平明显升高，与正常组比较差异均有统计学意义（P〈0．05）。给予不同剂量姜黄素后能逆转上述行为学改变及大脑皮层、回肠的5-HT、BDNF水平变化，其中姜高组与IBS模型组比较差异均有统计学意义（P〈0．05）。丙咪嗪组效果与姜高组类似。结论IBS大鼠脑肠内5-HT、BDNF表达异常说明IBS的发生存在脑肠改变的物质基础。姜黄素可能是通过调节5-HT、BDNF表达来改善IBS模型大鼠的行为学变化。Objective To investigate the pathogenesis of irritable bowel syndrome （IBS） from the aspect of brain-gut interaction and find out the efficacy and mechanism of curcumin on IBS. Methods Forty-eight rats were chosen in our study, and divided into 6 groups （n=8）： normal group, IBS model group, IBS＋curcumin treatment groups （10, 20 and 40 mg/kg of curcumin via intragastric administration 1 h before model inducement） and IBS＋imipramine treatment group （10 mg/kg, via intraperitoneal injection 30 min before model inducement）; IBS rat models were established by chronic and acute stress; treatments in each group were given for a consecutive 21 days. The depression-like behaviors and gut hypersensitivity of the rats in all the groups were detected. The expressions of 5-hydroxytryptamine （5-HT） and brain-derived neurotrophic factor （BDNF） in the cerebral cortex and ileum were determined by high performance liquid chromatography （HPLC） and Western blotting, respectively. Results The sucrose consumption of IBS rat had been significantly reduced and the intestinal viscera sensitivity was obviously elevated in the IBS model group as compared with those in the normal group （P〈0.05）; as compared with those in the normal control group, the expression of 5-HT significantly decreased in the cerebral cortex of IBS model group （P〈0.05）, but：opposite presentations were noted in the ileum （P〈0.05）. As compared with that in the normal group, the BDNF expression in the brain frontal cortex was significantly decreased but obviously increased in the ileum （P〈0.05）. Highdoses of curcumin （40 mg/kg treatment groups） improved the behavior oflBS rats, and reversed the levels of 5-HT in the cerebral cortex and ileum, with significant difference （P〈0.05）; besides, they could significantly reverse the BDNF level in the cerebral cortex and ileum, with significant difference （P〈 0.05）. Conclusion IBS rat models have abnormal expressions of 5-HT and 5-HIAA, and BDNF in the br

关 键 词：姜黄素 肠易激综合征 5-羟色胺 脑源性神经营养因子 

分 类 号：R285.5[医药卫生—中药学]