鸡传染性法氏囊病病毒流行株VP1部分序列及VP2高变区序列分析  

Sequence analysis of VP1 partial sequence of infectious bural disease virus epidemic strains

作　　者：熊忠贤[1] 何秀苗[1] 杨霖[1] 戴书剑[1] 韦平[2] 刘红全[1] 

机构地区：[1]广西民族大学化学与生物转化过程重点实验室,广西南宁530006 [2]广西大学,广西南宁530005

出　　处：《中国预防兽医学报》2013年第5期419-422,共4页Chinese Journal of Preventive Veterinary Medicine

基　　金：广西自然科学基金(2012GXNSFAA053060;桂科自0728058);广西民族大学研究生教育创新计划项目(Gxun-chx2012082)

摘　　要：为了对鸡传染性法氏囊病病毒(IBDV)的13个地方分离株与3个商品疫苗株的VP1-b基因(756 bp～1 522 bp)进行同源性和遗传进化分析,本研究对其进行RT-PCR扩增和序列测定。结果表明,获得的VP1-b基因长度均为767 bp;13个分离株VP1-b节段核苷酸和氨基酸同源性分别为90.7%～100%和72.9%～100%,其中10株超强毒分离株与经典株、弱毒株、疫苗株的同源性较高,核苷酸和氨基酸同源性分别为95.7%～100%和94.5%～100%,所有12个超强毒分离株与超强毒参考病毒株的同源性均较低,核苷酸同源性仅为68.2%～79.2%;在遗传进化分析中,所有病毒株被分为3个分支,与相应病毒株的基于IBDV-VP2高变区(vVP2)序列的遗传进化树比较并结合同源性分析结果表明,除了弱毒株HUN0804之外,其它12个分离株可能均为重组病毒。据此推测,基因重组可能是目前IBDV流行的新趋势。The VPI-b fragment （756 bp to 1,522 bp） of 13 local isolates and 3 vaccine strains of infectious bursal disease virus （IBDV） were amplified by RT-PCR and sequenced, their sequences homology and phylogenetic analysis were performed. The results showed that the length of the VPI-b fragment of all the IBDVs studied was 767 bp. Nucleotide and amino acid sequences analysis showed that the homology between 13 IBDV isolates were 90.7% to 100 % and 72.9% to 100%, respectively. Ten very virulent （vv） IBDV isolates shared higher homology with classical strain, the vaccine strains, and the classical strain （identities of 95.7% to 100% in nucleotide level and 94.5% to 100% in amino acid level）. All the 12 wlBDV isolates showed low identity to the vvlBDV reference strains （identities of 68.2 % to 79.2% in nucleotide level）. In the phylogenetic analysis, all the IBDVs were grouped into 3 large clusters, and when compared to the phylogenetic tree based on the hyper-variable region of IBDV-VP2 gene （vVP2）, the genome of 12 vvlBDV isolates might have been reassorted, result in recombinant virus. We speculate that recombinant IBDV might be a new trend in IBDV epidemiology.

关 键 词：传染性法氏囊病病毒 VP1 序列分析 遗传进化分析 

分 类 号：S852.65[农业科学—基础兽医学]