地塞米松对慢性哮喘小鼠肺组织中白细胞介素21、磷酸化信号转导及转录激活因子3的干预作用  

Effect of dexamethasone on expression of interleukin-21 and phospho-STAT3 in a murine model of chronic asthma

作　　者：宫静[1] 罗雅玲[1] 张旃[2] 王慰盈[1] 李俊红[1] 

机构地区：[1]南方医科大学南方医院呼吸内科,广东广州510515 [2]武汉大学人民医院呼吸内科,湖北武汉430060

出　　处：《南方医科大学学报》2013年第5期742-745,共4页Journal of Southern Medical University

基　　金：国家自然科学基金(30770936);中华医学会慢性呼吸道疾病专项基金(08020460124)~~

摘　　要：目的研究慢性哮喘小鼠肺组织白细胞介素21(IL-21)、磷酸化信号转导及转录激活因子3(p-STAT3)表达变化,探讨地塞米松对小鼠肺组织IL-21、p-STAT3表达的影响。方法 SPF级BALB/C雌性小鼠33只,随机分为对照组、哮喘组、地塞米松治疗组,每组11只。卵清白蛋白(OVA)致敏和激发建立哮喘小鼠模型;肺泡灌洗液进行细胞计数和分类;HE染色观察气道炎症程度;免疫组化法观察IL-21和p-STAT3在小鼠肺组织的表达;免疫蛋白印迹法分析小鼠肺组织中IL-21、p-STAT3蛋白的表达。结果哮喘组肺泡灌洗液中的细胞总数和嗜酸性粒细胞计数较对照组和地塞米松组明显增加(P<0.01);哮喘组小鼠肺组织IL-21蛋白、p-STAT3蛋白的表达高于对照组(P<0.05),地塞米松组IL-21蛋白、p-STAT3蛋白的表达低于哮喘组(P<0.05)。结论地塞米松抑制了慢性哮喘小鼠模型IL-21、p-STAT3的表达,IL-21/STAT3细胞信号通路可能为地塞米松治疗哮喘的作用机制之一。Objective To investigate the effects of dexamethasone on the expression of interleukin-21 （IL-21） and phospho- STAT3 （p-STAT3） in a murine model of chronic asthma. Methods Thirty-three female BALB/C mice were randomly divided into control group, asthmatic group and dexamethasone-treated group （n=11）. In the latter two groups, asthmatic models were established by ovalbumin administration. The cells in the broncho-alveolar lavage fluid （BALF） were counted and classified. The airway inflammation was evaluated by HE staining, and the expressions of IL-21 and p-STAT3 in the lung tissue were detected by immunohistochemistry and Western blotting. Results The total cell number and lymphocyte number in the BALF were significantly higher in asthmatic group than in the control group and dexamethasone group （P〈0.01）. Compared with the control group, the asthmatic group showed significantly increased protein expressions of IL-21 and p-STAT3 （P〈0.05）, which were reduced by dexamethasone intervention （P〈0.05）. Conclusion Dexamethasone can inhibit the expression of IL-21 and p-STAT3 in the murine model of chronic asthma, suggesting the importance of IL-21/STAT3 signaling pathway in the therapeutic mechanisms of dexamethasone for asthma.

关 键 词：哮喘 白细胞介素21 磷酸化信号转导及转录激活因子3 地塞米松 

分 类 号：R562.25[医药卫生—呼吸系统]