根据生物学变异度确定血液学检测下限的研究  

Use biological variation to value the lower detect limit of hematology and platelet aggregation test

作　　者：吴俊[1] 赵洁[1] 宋英[1] 赵磊[1] 赵京忠[1] 

机构地区：[1]北京大学人民医院检验科,北京100044

出　　处：《中国实验诊断学》2013年第5期841-843,共3页Chinese Journal of Laboratory Diagnosis

基　　金：教育部高等院校博士学科点专项科研基金;课题号:20110001120088

摘　　要：目的确定血常规分析,血小板聚集检验最低检测下限范围。方法 Sysmex2100血液分析仪检测血常规,将标本倍比稀释后重复检测10次样本,计算变异系数CV值,做曲线及计算方程,以1/2生物变异度规定为允许CV值,该CV值代入方程为检测低限。对于血小板聚集,稀释后检测血小板聚集,在低于平台区的范围定位检测下限。结果白细胞检测下限为0.11×109/l,红细胞检测下限为0.14×1012/l,红细胞压积检测下限3.03%,血小板检测下限为15.31×109/l。血小板聚集需要最低的血小板数量为200×109/l。结论以生物变异度为基础的CV来确认仪器检测下限,方法可行,且与临床实际情况相符合。可作为确定仪器检测下限的方法。Objective To detect the lower limit of hematology,platelet aggregation. Methods we use sysmex 2100 hematology analyzer blood cell account;Chrono-log platelet function instrument test platelet aggregation rate. After di- luting samples,we retest the sample 10 times,plot X-axis with concentration,Y-axis with CV; ,calculated the equa- tion. We set 1/2 biological variation as standard CV to get the lower limit of detection. As for platelet aggregation, the concentration below the plateau is the lower limit concentration. Results Low limit detection of white blood cell,red blood cell,hematocrit,platelet is 0.11×10^9/l, 0.14×10^12/l,3.03%, 15.31×10^9/l, respectivly. The low concentration of platelet aggregation is 200×10^9/l. Conclusion Use biological variation to set the lower limit of detection is practicable. It fit with clinical situation. The 1/2 biological variation could be the standard of lower limit of detection evaluation.

关 键 词：血常规分析 血小板聚集 检测下限 生物变异度 

分 类 号：R446.11[医药卫生—诊断学]