高效液相色谱法测定眼氨肽滴眼液中醋酸氯己定的含量  

Determination of chlorhexidine acetate in ocular extractives eye drops by HPLC

作　　者：周朝东[1] 黄哲甦[1] 

机构地区：[1]天津市药品检验所,天津300070

出　　处：《天津药学》2013年第2期15-17,共3页Tianjin Pharmacy

摘　　要：目的:建立高效液相色谱法测定眼氨肽滴眼液中防腐剂醋酸氯己定的含量。方法:采用高效液相色谱法,色谱柱为welch materials AQ-C18柱(150 mm×4.6 mm,5μm),流动相为0.02 mol/L磷酸二氢钾(磷酸调节pH至2.5)-乙腈(70∶30),流速为1.0 ml/min,柱温为40℃,检测波长为254 nm。结果:醋酸氯己定在0.05～0.15 mg/ml范围内,浓度与其峰面积呈良好线性关系(r=0.9999,n=5),平均回收率为99.84%,RSD=0.42%(n=9)。结论:所用方法简单、快速、专属性强、重现性好,可用于眼氨肽滴眼液中防腐剂醋酸氯己定的测定。Objective ： to establish a HPLC method for the determination of ehlorhexidine acetate in ocular extraetives eye drops. Methods： The Cls column was used and the mobile phase consisted of 0.02 mol/L potassium dihydrogen phosphate（adjust to the pH 2.5 with phosphoric acid ） - acetonitriie （ 70：30 ） ; The flow rate was 1.0 ml/min; The column temperature was 40℃; the detection wavelength was 254 nm. Results ： the linear range of Chlorhexidine acetate was 0.05 - 0.15 mg/ml （ r = 0. 9999, n = 5 ） , the average recovery rate was 99.8% （RSD = 0.42% ）. Conclusion： the method is simple, rapid, strong specifity and accurate with good reproducibility, which is suitable for the content determination of chlorhexidine acetate in ocular extractives eye drops

关 键 词：高效液相色谱法 眼氨肽滴眼液 醋酸氯己定 含量测定 

分 类 号：R927.2[医药卫生—药学]