糖尿病大鼠肾脏纤维化与CTGF表达的相关性  

Correlation between renal fibrosis and protein expression of renal connective tissue growth factor in rats with diabetic nephropathy

作　　者：张树华[1] 孙冬梅[2] 马琳[1] 李静[3] 谢芳[1] 杨华[1] 孙萌[1] 薛瑞凤[4] 刘洋[1] 张俊玲[1] 

机构地区：[1]河北联合大学附属医院超声科,唐山063000 [2]河北联合大学研究生院 [3]唐山市妇幼保健院超声科 [4]唐山市工人医院分院内分泌科

出　　处：《中国循证心血管医学杂志》2013年第2期190-193,共4页Chinese Journal of Evidence-Based Cardiovascular Medicine

摘　　要：目的探讨糖尿病大鼠肾脏纤维化与结缔组织生长因子(CTGF)表达的关系。方法应用腹腔注射链脲佐菌素(STZ)的方法诱导糖尿病大鼠模型,共成功造模23只,其中18只按照饲养时间随机分为3组;4周(n=6)、12周(n=6)、24周(n=6)],同时设置18只大鼠作为对照组,分别饲养4周(n=6),12周(n=6)和24周(n=6),在相应时间点采用免疫组织化学法检测肾脏CTGF含量,同时进行肾脏病理检测并进行组间比较。结果糖尿病模型组CTGF蛋白表达在各时间点,随病程的进展,表达逐渐增高。第4周分别为(0.88±0.05)×103/μm2vs.(0.41±0.06)×103/μm2,第12周分别为(0.29±0.05)×103/μm2vs.(0.60±0.05)×103/μm2,第24周分别为(3.13±0.08)×103/μm2vs.(1.50±0.05)×103/μm2,差异均有统计学意义(P<0.05)。且CTGF的含量与肾脏间质血管周围胶原面积(PVCA)(r=0.89)、肾小球胶原沉积评分(GCDS)(r=0.87)、肾小管间质病变评分(TIDS)(r=0.76)、肾小球系膜基质指数(Ms/Gs)(r=0.82)(P<0.05)均呈正相关。结论糖尿病大鼠CTGF的高表达,可能与肾脏纤维化有关,检测肾脏CTGF含量可考虑作为评价肾脏纤维化的方法。Objective To investigate the relationship between renal fibrosis and protein expression of renal connective tissue growth factor （ CTGF ） in rats with diabetic nephropathy （ DN ） . Methods 18 rat models of DN was established by the intraperitonea injection of streptozotocin （ STZ ） , and then were divided into 4-week group （ n=6 ） , 12-week group （ n=6 ） and 24-week group （ n=6 ） , and 18 rats were included as control group, [which wew, randomly dividing 4-week group, 12-week group and 24-week group （ each n=6 ） ]. The level of renal CTGF was detected by immunohistoehemistry technique respectively at different time points, and pathological changes were observed simultaneously. Results The level of CTGF was higher in DN group than that in control group at all time points, and increased gradually with the development of the disease （ P〈0.05 ） . The level of CTGF was （ 0.88 -± 0.05 ） x 103/I,L m： vs. （ 0.41 ± 0.06 ） 10^3/μm^2on the 4th week, （ 0.29 ± 0.05 ）10^3/μm^2vs. （0.60±0.05） xl03/Ixm2onthe 12thweek, and （3.13_±0.08） 10^3/μm^2vs. （1.50±0.05） 10^3/μm^2 on the 24th week （ P〈0.05 ） . The level of CTGF was positively related to the correlation coefficients of renal fibrosis indexes including PVCA （ r=0.89 ） , GCDS （ r=0.87 ） , TIDS （ r=0.76 ） and Ms/Gs （ r=0.82, P 〈0.05 ） . Conclusion The expression of CTGF may be related to renal fibrosis, so terting of CTGF can be taken as an important method for prognose renal fibrosis.

关 键 词：结缔组织生长因子 肾脏纤维化 糖尿病肾病 大鼠 

分 类 号：R544.1[医药卫生—心血管疾病]