量子点对小鼠卵巢颗粒细胞的毒性  

Toxicity of Quantum Dots on Mouse Ovarian Granulosa Cells

作　　者：谢向毅[1] 朱小妹[1] 滕欢[1] 翟鹏[2] 王光笋[2] 林苏霞[2] 王晓梅[2] 许改霞[1] 牛憨笨[1] 

机构地区：[1]深圳大学光电工程学院教育部/广东省光电子器件与系统重点实验室,广东深圳518060 [2]深圳大学医学院深圳市生物医学工程重点实验室,广东深圳518060

出　　处：《中国激光》2013年第5期120-125,共6页Chinese Journal of Lasers

基　　金：国家973计划(2012CB85802);国家自然科学基金(3090035;61235012);深圳市基础研究项目(JC201005280500A)资助课题

摘　　要：研究了CdTe量子点对体外培养小鼠卵巢颗粒细胞的毒性。采用荧光光度计测量了该量子点在DMEM培养基、M1640培养基和磷酸盐缓冲液中的光谱稳定性,分别利用Hoechst32258染色和CCK-8试剂对量子点处理颗粒细胞的凋亡情况和存活率进行检测。结果表明量子点在DMEM培养基中稳定性比其他溶液好,24h内量子点的发射峰波长无明显偏移,荧光强度仅下降9.8%;Hoechst32258凋亡染色发现细胞核凋亡量随着量子点浓度的升高而增加,CCK-8检测也得到一致的结论。该研究表明,在低浓度下量子点对颗粒细胞的形态、功能无影响,随着量子点在颗粒细胞质中蓄积量的增加,颗粒细胞凋亡率也明显升高。本研究为量子点在活体研究中的安全应用提供了生殖毒性参考。This study investigates the toxicity of CdTe quantum dots （QDs） on mouse ovarian granulosa cells in vitro. The fluorescence stabilities of QDs in DMEM culture medium, M1640 culture medium and phosphate buffer solution （PBS） are measured by fluorometer, respectively. Apoptosis is observed by chromatin staining with Hoechst32258 and cell viability is detected by cell counting kit-8 （CCK-8） assay after the granulosa cells are treated with QDs. The results demonstrate that the fluorescence stability of QDs in DMEM culture medium is better than that in the other media, the QDs fluorescence emission spectrum in it does not shift and the intensity decreases only 9.8 %. It is found that the apoptosis of granulosa cells treated with QDs is dosage-dependent by the Hochest32258 staining, which is confirmed by the CCK-8 assay. The research demonstrates that the QDs at lower concentration have not affected the morphology and function of granulosa cells in vitro. However, with the increase of accumulation of QDs inside cytoplasm, the apoptosis increases significantly. This study offers very useful information of reproductive toxicity of QDs to guide the safe application of QDs in vivo.

关 键 词：生物技术 量子点 卵巢颗粒细胞 凋亡染色 生殖毒性 

分 类 号：TN[电子电信]