rDNA-ITS2序列作为中国按蚊分子鉴别特征的评述：I按蚊亚属  

THE SECOND INTERNAL TRANSCRIBED SPACER OF NUCLEAR RIBOSOMAL DNA AS A MOLECULAR IDENTITY FOR CHINESE ANOPHELINE TAXONOMY： I SUBGENUS ANOPHELES SPECIES

作　　者：伍桐[1,2] 马雅军[1] 

机构地区：[1]第二军医大学基础医学部病原生物学教研室,上海200433 [2]第二军医大学研究生管理大队,上海200433

出　　处：《寄生虫与医学昆虫学报》2013年第1期16-24,共9页Acta Parasitologica et Medica Entomologica Sinica

基　　金：国家科技重大专项课题（2012ZX10004219-010）;第二军医大学大学生创新能力培养基金（MS2012018）

摘　　要：对核糖体DNA第2内转录间隔区（rDNA-ITS2）序列作为中国按蚊属按蚊亚属蚊种鉴别的分子特征进行评价。笔者整理和分析了GenBank数据库中注册的所有中国记录的按蚊亚属蚊种的rDNA—ITS2序列，并应用MEGA软件计算其在种内和种间的差异性（P距离）。截止2012年10月，GenBank中已注册ITS2序列共584条，隶属按蚊亚属蚊20种（包括同物异名）、待定种5种和杂交种1个。计算结果显示，ITS2序列在种内的变异性小于1％，种间的差异性范围为1．2％（凉山按蚊与昆明按蚊）-69．5％（待定种YM．2003a与须喙按蚊）。另外，发现少数注册的序列在种内差异性较大，在比对序列时需谨慎。结果提示rDNA-ITS2序列在本研究按蚊中具有良好的种内保守性和种间解析度。To evaluate the ribosomal DNA second internal transcribed spacer （rDNA-ITS2） sequences as a identification identity of Chinese genus Anopheles subgenus Anopheles mosquito species. The rDNA-ITS2 sequences of Chinese subgenus Anopheles mosquito species registered in the GenBank database were downloaded and organized. The intraspecific variation and interspecific difference （p-distance） were estimated by MEGA software. By October 2012, there were 584 of ITS2 sequences registered on GenBank, which belong to 20 species （including synonymy） , 5 unnamed species and 1 hybrid. The value of intraspecific p-distance was less than 1%, and the range of interspecific p-distance as from 1.2% （An. liangshaneneis with An. kunmingensis） to 69.5% （An. sp YM-2003a with An. barbirostris）. In addition, intraspecific variation of a few sequences was considerable, which need to be careful in the blast searching. The result showed that the rDNA-ITS2 sequence has a good conservation and interspecific resolution in this anopheles mosquito.

关 键 词：RDNA-ITS2 按蚊亚属 鉴别 评述 

分 类 号：Q969.442.2[生物学—昆虫学]