高糖对大鼠肾小球系膜细胞增殖、MCP-1表达及NF-κB活化的影响  

Effects of high glucose on rat glomerular mesangial cells proliferation, MCP-1 expression and NF-κB activation

作　　者：贾倩倩[1] 王建成[1] 赵艳[1] 陈斯佳[1] 龙海波[1] 

机构地区：[1]南方医科大学珠江医院肾内科,广东广州510280

出　　处：《热带医学杂志》2013年第4期382-384,共3页Journal of Tropical Medicine

基　　金：国家自然科学基金(81072848);广东省科技计划项目(2009B060700054;2011B061300014)

摘　　要：目的探讨高糖环境对大鼠肾小球系膜细胞增殖、单核细胞趋化蛋白-1(MCP-1)的表达及NF-κB活化的影响。方法将体外培养大鼠肾小球系膜细胞(MC)分为4组:正常对照组(含5.6 mmol/L葡萄糖)、高糖1组(含15 mmol/L葡萄糖)、高糖2组(含20 mmol/L葡萄糖)和高糖3组(含25 mmol/L葡萄糖)。MTT法检测细胞的增殖情况,ELISA法检测MCP-1的表达,Western blotting法检测IκBα以反映NF-κB的活性。结果与正常对照组比较,高糖环境下24 h后细胞增殖明显,MCP-1表达明显增强(P<0.01)。高糖刺激30 min后,NF-κB结合蛋白IκBα明显降解,提示高糖可诱导MC NF-κB活化。结论高糖环境下大鼠肾小球系膜细胞的增殖及炎症因子MCP-1的高表达可能是通过NF-κB的活化来实现的。Objective To explore the effect of high glucose on cells proliferation,monocyte chemoattractant protein-1 （MCP- 1） expression and NF-KB activation in rat mesangial cell（MC）. Methods The in vitro culture of rat glomerular mesangial ceils were divided into four groups： normal glucose group （glucose concentration of 5.6 retool/L）, high glucose group 1 （glucose concentration of 15 mmol/L）, high glucose group 2 （glucose concentration of 20 mmol/L）, and high glucose group 3 （glucose concentration of 25 retool/L）. MTI＇ method was used to detect cell proliferation. MCP-1 in the supernatant was measured by ELISA. IKBct expression was detected by Western blotting method to reflect the NF-KB activation. Results The MC proliferation and MCP-I expression were significantly increased after 24 h in high glucose groups （P〈0.01）. The expression of NF-KB-binding protein IKBct was obviously declined after 30 min of high glucose treatment, and indicated that high glucose could induce the NF-KB activation in MC. Conclusion High glucose could stimulate rat glomerular mesangial cell proliferation and promoted inflammation factor MCP-1 expression may be through the NF-KB activation.

关 键 词：高糖 糖尿病.肾病 单核细胞趋化蛋白-1 

分 类 号：R587.2[医药卫生—内分泌]