2008至2011年上海单中心住院腹泻儿童轮状病毒基因型别流行特征  

Epidemical characteries of group A Rotavirus genotypes in children hospitalized with diarrhea in Shanghai, during 2008-2011

作　　者：卢丽娟[1] 钟华清[1] 苏犁云[1] 曹凌峰[1] 徐梦华[1] 徐锦[1] 

机构地区：[1]复旦大学附属儿科医院儿科研究所,上海201102

出　　处：《中国循证儿科杂志》2013年第2期98-104,共7页Chinese Journal of Evidence Based Pediatrics

基　　金：新世纪优秀人才计划:NECT-09-0320;上海市公共卫生优秀学科带头人计划:GWDTR201221

摘　　要：目的监测并分析上海单中心住院腹泻儿童A组轮状病毒(RV)基因型别的变化特征,为RV性腹泻的防治提供基础数据和理论依据。方法收集2008年1月至2011年12月入住复旦大学附属儿科医院<5岁的腹泻患儿粪便标本,RV抗原阳性者345份,采用套式多重RT-PCR法进行RV的基因分型。结果①G基因型:2008至2010年以G3型为主,检出率分别为49.2%、44.6%和78.0%;G9型的流行呈上升趋势,成为2011年最主要的流行型别(51.1%);G1型在4年中均有检出(8%～20%);G2型少见,G4型未检出。G混合型中,以G3+G9型为主,其次为G3+G1型,还检出4例3种G基因型的混合。②P基因型:2008、2010和2011年均以P[8]型为主,检出率分别为55.6%、60.0%和68.1%,2009年以P混合基因型(43.2%)为主;P[4]型在4年均有流行;P[6]、P[9]型少见,仅在混合感染中检出P[10]型。P混合型中,以P[8]+P[4]型为主,其次为P[8]+P[10]型。③P[8]G3型是2008至2011年最主要的RV流行株(24.3%),其次为P[8]G9型及PmG3型。2011年P[8]G9型跃升为最主要的流行型别(40.5%)。结论与2001至2007年相比,2008至2011年上海地区A组RV基因型出现了新的流行特点,G1型及G9型的流行呈上升趋势,G3的流行有所下降,各种混合型别多见。对RV基因型保持系统性的连续监测对RV疫苗在上海的应用是必要的。Objective This study aimed to analyze the dynamic characteristics of group A rotavirus genotypes. Methods A total of 345 rotavirus-positive stool specimens were collected from inpatients under 5 years old hospitalized with acute gastroenteritis in Children＇s Hospital of Fudan University from January 2008 to December 2011. Each fecal specimen was tested for rotavirus with a commercial enzyme immunoassay, and genotypes were characterized using multiplex nested reverse transcription polymerase chain reaction （RT-PCR）. Results G genotypes： The predominant G genotype was G3 which accounted for 49.2% , 44.6% and 78. 0% respectively in the year of 2008, 2009 and 2010. The prevalence of （;9 was on an upward trend from 2008 to 2011, and dominated as the most common type in 2011 （51.1%）. G1 was detected each year with a varying prevalence of 8% -20%. （;2 was rarely seen and G4 was not detected during the four years. Mixed G infections with G3 ＋ （;9 were the most prevailing G-mixed types, followed by G3 ＋ G1. Otherwise, mixed-G infections with three different G genotypes were detected in 4 cases. P genotypes ： P[ 8 ] genotype was the dominant strain with prevalence of 55.6% , 60.0% and 68.1% respectively in the year of 2008, 2010 and 2011, whereas mixed P genotype was dominant in 2009（43.2% ）. P[4] strains were prevalent in four years in a volatility way. P [6] and P[9] could also be found as minor types. Interestingly, P [ 10 ] genotype was seen only in combination with P[ 8 ]. The major mixed P infection was genotype of P [ 4] ＋ P [ 8 ], and P [ 8 ] ＋ P[ 10 ] was assigned as follows. A high prevalence of P [ 8 ] G3 genotype was determined to be the major rotavirus genotype combination （24.3%） in Shanghai from 2008 to 2011 and the other prevalent genotypes included P[ 8 ] G9 and P [ m ] G3. P [ 8 ] G9 strains jumped as the most predominant type in 2011 （ 40.5% ）. Conclusions Compared with the data from the year of 2001 -2007, this study shows new epidemiological characteristics of gr

关 键 词：婴幼儿 轮状病毒 腹泻 基因型 

分 类 号：R725.1[医药卫生—儿科]