肠吉泰对腹泻型肠易激综合征大鼠下丘脑、结肠P物质mRNA表达的影响  

Effect of Changjitai decoction on expression of substance P mRNA in the colonic mucosa and hypothalamus of rats with diarrhea-predominant irritable bowel syndrome

作　　者：丛军[1] 蔡淦[1] 林江[1] 张正利[1] 

机构地区：[1]上海中医药大学附属曙光医院消化科,上海市200021

出　　处：《世界华人消化杂志》2013年第13期1234-1238,共5页World Chinese Journal of Digestology

摘　　要：目的:观察肠吉泰对腹泻型肠易激综合征(irritable bowel syndrome,IBS)大鼠下丘脑、结肠P物质mRNA表达的影响,探讨其治疗机制.方法:♂SD大鼠60只,随机分为正常组、模型组、肠吉泰低、中、高剂量组和得舒特组除正常组外各组大鼠予束缚应激加大黄泻下及结直肠扩张的造模方法,并予以相应药物治疗.实验过程中观察各组大鼠大便性状和次数;大鼠处死后取下丘脑及结肠标本用RT-PCR半定量检测P物质mRNA的表达.结果:与正常组比较,模型组大鼠的下丘脑、结肠P物质mRNA表达均有升高(0.988±0.192vs 0.606±0.151,P<0.01;1.042±0.293 vs 0.768±0.116,P>0.05);与模型组比较,肠吉泰治疗组大鼠P物质mRNA表达下调(下丘脑:0.678±0.103,0.733±0.103 vs 0.988±0.192,P<0.01;结肠:0.546±0.278,0.500±0.260vs 1.042±0.293P<0.01;0.731±0.199 vs 1.042±0.293,P<0.05)得舒特组、肠吉泰高、中剂量组大便次数及性状明显改善(14、13、17vs 24,P<0.05),且肠吉泰与得舒特组比较疗效无显著差异.结论:肠吉泰通过调节大鼠下丘脑、结肠P物质mRNA表达量,改善内脏痛觉敏感状态,及其对束缚应激反应导致肠动力的紊乱.AIM：To detect the expression of substance P（SP） mRNA in the colonic mucosa and hypothalamus of rats with diarrhea-predominant irritable bowel syndrome（IBS-D）,and to assess the therapeutic effect of traditional Chinese medicine Changjitai decoction on visceral hypersensitivity in rats with IBS-D.METHODS：Sixty male Sprague-Dawley rats were equally and randomly divided into six groups：normal group,model control group,dicetel group,low-,medium-,and high-dose Changjitai groups.IBS-D was induced by restraint stress for 2 wk combined with intragastric administration of 2 mL of Radix Rhizoma Rhei leachate from the third day in rats of all groups except the normal group.After treatment with corresponding drug for 2 wk,ratsin the model group and treatment groups underwent colorectal distention immediately and then killed.The changes in defecation were monitored by measuring fecal pellets.The expression of SP mRNA in the hypothalamus and colon was detected by RT-PCR.RESULTS：In the model control group,the frequency of defecation increased obviously compared to the normal group,and the characteristics of stools deteriorated simultaneously.The expression of SP mRNA in the hypothalamus and colon was significantly increased in the model group compared to the normal group（0.988±0.192 vs 0.606±0.151,P〈0.01;1.042±0.293 vs 0.768±0.116,P〈0.05）.After treatment with Changjitai decoction or diceter,the frequency of defecation and the characteristics of stools ameliorated significantly（13,17,14 vs 24,P〈0.05）.There were no significantly differences in the above parameters between the Changjitai groups and dicetel group.SP mRNA levels were significantly reduced in the Changjitai decoction groups compared to the model control group（hypothalamus：0.678±0.103,0.733±0.103 vs 0.988±0.192,P〈0.01;colon：0.546±0.278,0.500±0.260 vs 1.042±0.293,both P〈0.01;0.731±0.199 vs 1.042±0.293,P〈0.05）.CONCLUSION：Changjitai decoction relieves diarrhea symptoms,ameliorates the stress state,inhibits bowel mo

关 键 词：腹泻型肠易激综合征 内脏高敏感 P物质 肠吉泰 

分 类 号：R285.5[医药卫生—中药学]