β-榄香烯对肿瘤细胞上清液诱导的大鼠骨髓来源内皮祖细胞增殖与凋亡的影响  

Effect of β-elemene on proliferation and apoptosis of rat bone marrow derived endothelial progenitor cells induced by tumor cells supernatant

作　　者：宋颖[1,2] 刘云鹏[3] 杨向红[2] 

机构地区：[1]新乡医学院病理学教研室,河南新乡453003 [2]中国医科大学附属盛京医院病理科,辽宁沈阳110004 [3]中国医科大学第一附属医院肿瘤内科,辽宁沈阳110001

出　　处：《新乡医学院学报》2013年第5期368-370,共3页Journal of Xinxiang Medical University

摘　　要：目的观察β-榄香烯对肿瘤细胞上清液诱导的大鼠骨髓来源内皮祖细胞(EPCs)增殖与凋亡的影响,探讨β-榄香烯对EPCs参与肿瘤血管形成过程的作用。方法原代培养大鼠骨髓来源EPCs经胃腺癌细胞株SGC-7901培养上清液诱导EPCs 12h后,分为浓度效应组(β-榄香烯0、5、10、20mg.L-1干预48h)和时间效应组(20mg.L-1β-榄香烯干预0、12、24、48h)。检测各组EPCs的增殖和凋亡率。结果随着β-榄香烯剂量的增加和作用时间的延长,EPCs的增殖显著下降(P<0.05),当β-榄香烯浓度为5mg.L-1及作用时间为12h时,EPCs凋亡率变化不明显,当β-榄香烯浓度增加至10mg.L-1及作用时间延长为24h时,EPCs凋亡率显著上升(P<0.05)。结论β-榄香烯通过诱导EPCs的凋亡抑制其增殖,并可削弱EPCs在肿瘤血管发生中的作用。Objective To investigate the effect of β-elemene on the proliferation and apoptosis of rat bone marrow de- rived endothelial progenitor cells（EPCs） induced by tumor cells supernatant,and discuss the effect of β-elemene on tumor an- giogenesis which EPCs participated in. Methods Rat bone marrow derived EPCs which were primarily cuhured were cultured with SGC-7901 tumor cell supernatant for 12 h and treated with different concentration of β-elemene （0,5,10,20 mg . L^-1 ） for 48 h,or treated with 20 mg · L^-1 β-elemene for different time（0,12,24,48 h）. The proliferation and apoptosis rate of EPCs in each group were determined. Results With the increasing of the β-elemene dose and action time, proliferation of EPCs decreased significantly （ P 〈 0. 05 ）. The apoptosis rate didn＇t change significantly when the β-elemene dose was 5 mg .L^-1 and the action time was 12 h,but increased singificantly when the β-elemene dose was 10 mg ~ L-1 and the action time was 24 h（P 〈 0. 05）. Conclusion β-elemene can decrease the effect of EPCs on tumor angiogenesis through inducing apoptosis and inhibiting proliferation of EPCs.

关 键 词：内皮祖细胞 肿瘤细胞上清液 Β-榄香烯 增殖 凋亡 

分 类 号：R329.2[医药卫生—人体解剖和组织胚胎学]