十二烷基硫酸钠诱导银屑病动物模型的建立  

Establishment of a psoriasis animal model using SDS

作　　者：张海婧[1] 周琬琪[1] 张翼[1] 金晶[1] 陈晓光[1] 

机构地区：[1]天然活性药物与功能国家重点实验室中国医学科学院药物研究所

出　　处：《武警医学》2013年第5期373-375,共3页Medical Journal of the Chinese People's Armed Police Force

基　　金：国家自然科学基金青年基金项目(81001443)

摘　　要：目的建立一种简单、快速筛选银屑病治疗药物的模型。方法应用10%十二烷基硫酸钠(sodium dodecyl sulfate,SDS)涂抹模型组小鼠背部皮肤,对照组小鼠涂抹蒸馏水,观察两组皮肤组织的改变。结果经过10% SDS涂抹7 d后的模型组小鼠皮肤可见明显银屑病样体征,如皮肤增厚[(0.87±0.33)mm vs(0.60±0.02)mm]、红肿和皮下血管增生等。病理检测证明模型组小鼠皮肤出现角化过度和角质层增厚,真皮层和皮下层可见毛细血管扩张和充血等银屑病样改变。West-ern-blot结果证明,血清中的PKCβ、IL-1β、TNF-α、IL-23等细胞因子表达上调。结果显示SDS所诱导的小鼠模型能够反映银屑病的某些特征。结论利用10%SDS涂抹小鼠皮肤可建立银屑病动物模型,方法简单、快速。Objective To establish a simple, rapid psoriasis model. Methods 10% SDS was applied to the skin of test group ,while distilled water was applied to the skin of control group. Results After 10% SDS was applied for 7 d, psoriasis symptoms were produced on skin of test mice such as skin thickening, swelling and subcutaneous vascular proliferation, etc. Pathology detected hyperkeratosis and corneous layer thickening, visible blood capillary dilation and congestion in the dermis and subcutaneous layer of test mice skin, compared with control mice skin. Western blotting results showed expressions of PKC β, IL - 1 β, TNF alpha, and IL - 23 were upregulated. The results above showed that the SDS-induced mouse model could reflect the some characteristics of psoriasis. Conclusions A simple, rapid psoriasis animal model is established using 10% SDS.

关 键 词：银屑病 动物模型 十二烷基硫酸钠 

分 类 号：R332[医药卫生—人体生理学]