血清MicroRNA-122作为脓毒症诊断特异性标志物的研究  

Serum microRNA-122 as a specific marker of sepsis diagnosis

作　　者：邓婕[1] 王慧娟[2] 苏龙翔[2] 张鑫[1] 肖坤[1] 贾艳红[1] 解立新[1] 

机构地区：[1]解放军总医院呼吸科,北京100853 [2]南开大学医学院,天津300071

出　　处：《武警医学》2013年第5期383-386,共4页Medical Journal of the Chinese People's Armed Police Force

基　　金：国家自然科学基金(81170008);军队十二五面上课题(CWS11J094)

摘　　要：目的观察脓毒症患者血清中miRNA-122的表达量在病程中的动态变化情况,并探讨其与脓毒症严重程度的关系。方法选择52例脓毒症患者,其中一般脓毒症23例,重症脓毒症29例,23例健康人作为对照。采用荧光定量PCR(qRT-PCR)检测研究对象在入组第1,3,5,7,10,14天血清中miRNA-122表达量,并记录患者的基本临床信息。结果在脓毒症患者入组后14d的病程中,52例脓毒症患者和23例健康人群血清中的miRNA-122表达量在每个时间点比较,前者均比后者显著下降(P<0.01)。用入组第1天的miRNA表达量做ROC诊断分析得出,miRNA-122诊断脓毒症的特异性是95.7%,敏感性是53.8%。重症脓毒症组的血清miRNA-122表达量水平高于一般脓毒症组,并且在各个观测时间点两组表达量具有统计学差异(P<0.01)。结论血清中miRNA-122可能作为脓毒症诊断的特异性标志物,具有潜在的临床价值,并且miRNA-122在预警脓毒症患者疾病严重程度方面具有积极的临床意义。Objective To study the dynamic changes in serum miRNA-122 levels during sepsis and the correlation between serum miRNA-122 and sepsis severity. Methods Fifty-two sepsis patients （including 23 non-severe septic patients and 29 severe septic patients）and 23 healthy controls were recruited in the study. The expression levels of serum miRNA-122 were quantified by quantitative reverse transcriptase polymerase chain reaction assays （qRT-PCR）.Levels of serum miRNA-122 were detected on the 1 st, 3 rd, 5 th, 7 th, 10 th and 14 th day after their admissions in intensive care unit. All the patients clinical information was also recorded. Results After comparison, levels of serum miRNA-122 in sepsis patients on every observation time point were all significantly lower than those in healthy controls （P〈0.01）. The receiver operating characteristic curve was obtained and the area under curve was calculated on the levels of serum miRNA-122 on the first day of their admissions. When the cut-off point was set at 0.710, miRNA-122 had a specificity of 95.7% and a sensitivity of 53.8%. The expression levels of serum miRNA-122 in severe sepsis group were significantly higher than in sepsis group on each observation time point （P〈0.01）. Conclusions Serum miRNA-122 can used as a specific biomarker for diagnosis of sepsis. And miRNA-122 might be correlated with the organ injuries of septic patients.

关 键 词：脓毒症 微小RNA-122 微小RNA 诊断 

分 类 号：R593.9[医药卫生—内科学]