不同干预方式对WistarⅡ型糖尿病大鼠心肌组织氧化损伤指标、抗氧化酶及线粒体生物合成的影响  

Impact of Different Interventions on WistarⅡDiabetic Rat Myocardial Oxidative Damage Indicators,Antioxidant Enzymes and Mitochondrial Biogenesis

作　　者：朱健[1] 

机构地区：[1]中原工学院体育教学部,郑州450007

出　　处：《西安体育学院学报》2013年第3期334-339,共6页Journal of Xi'an Physical Education University

基　　金：河南省科技攻关项目(112102310694)

摘　　要：目的研究观察游泳训练,补充α-硫辛酸以及联合干预方式(游泳+α-硫辛酸)对Ⅱ型糖尿病大鼠心肌组织氧化应激指标、抗氧化酶及线粒体生物合成的影响,并初步探讨其内在作用机制。方法 60只Wistar大鼠在完成Ⅱ型糖尿病造模后,随机分成4组,即糖尿病对照组(CON)、α-硫辛酸(LA)组、游泳训练组(ST)和α-硫辛酸+游泳训练组(ST+LA)。ST组和ST+LA组进行游泳训练。训练方案为:60 min/d,周一至周五训练,共计8周。试剂盒测定大鼠心肌组织丙二醛(MDA)及过氧化脂(LPO)水平;Western blot方法检测线粒体抗氧化酶系MnSOD,Thi-ored-2,Prdx3,线粒体合成调节因子PGC-1α及二相酶HO-1,γ-GCL,NQO-1表达水平;透射电镜观察线粒体生物合成变化。结果 LA和ST均可显著性降低心肌组织中MDA含量,且联合作用效果存在放大效应(P<0.05);LPO的变化趋势与MDA相似。对于抗氧化酶的影响,ST既可有效提高线粒体抗氧化酶活性,又可显著性提高胞浆解毒酶蛋白表达(P<0.05);LA,除了显著性提高HO-1,γ-GCL外,对NQO-1及线粒体抗氧化酶的影响不具有统计学意义(P>0.05)。另外,ST和LA均可使线粒体数目增加,且在联合干预后,增强作用实现了优势互补(P<0.05)。结论联合作用方式引起的心肌保护作用与其提高抗氧化酶(实现单一干预方式对胞浆中抗氧化酶提高的叠加效应)相关。另外,联合作用在增加线粒体生物合成水平上存在优势增强效应。Objective This study was designed to observe the influences of swimming training （ST） , α - lipoic acid （LA） and both conjoined intervened modality （ST ＋ LA） on oxidative stress, antioxidant enzymes and mitochondrial biogenesis in myocardial tissue of Wistar diabetes Ⅱ rats, and to primary explore the associated mechanisms. Methods After diabetes Ⅱ models completion, 60 Wistar rats were randomly divided into four groups ： The control group （ CON）, the alpha - lipoic acid （LA） group, the swimming training group （ST） and the α - lipoic acid ＋ swimming training group （ ST ＋ LA）. ST group and ST ＋ LA group performed a swimming training. Exercise protocol ： 60 min/d ; from Monday to Friday ; total of 8 weeks. MDA and LPO were determined using kit ; MnSOD, Thioredoxin - 2, Peroxiredoxin 3, PGC - 1 α, HO - 1, γ- GCL, NQO - 1 were assayed with Western blot ; Mitoehondrial proliferation level was tested with EM. Results LA and ST could significantly decrease the contents of MDA and LPO; ST not only increases antioxidant enzymes in the mitochondria, but also elevates the protein levels of detoxifying enzymes in the cytoplasm （P 〈 0.05 ） ; LA, expect for increasing HO - 1, γ- GCL, has no obvious effect on others endogenous antioxidant proteins （P 〉 0.05）. In addition, either ST or LA could increase mitochondrial content. Importantly, after intervened with jointed modality, increased effect induced by each separate model was noticeably enhanced （ P 〈 0.05 ）. Conclusions Jointed model - mediated myocardial protection was related to its enhancement of antioxidant enzymes and improvement of mitochondrial biogenesis.

关 键 词：Ⅱ型糖尿病 游泳训练 Α-硫辛酸 氧化应激 线粒体增殖 

分 类 号：G804.7[文化科学—运动人体科学]