HPLC法测定大青根中腺苷和丁香树脂酚葡萄糖苷的含量  

Determination of Adenosine and Syringaresinol-4-O-β-D-glucopyranoside in Root of Clerodendron cyrtophyllum Turcz by HPLC

作　　者：李艳[1,2] 田星[1,2] 安晔[1] 何进[1] 赵庆春[1] 

机构地区：[1]沈阳军区总医院药剂科,沈阳110840 [2]沈阳药科大学药学院

出　　处：《中国药师》2013年第5期679-681,共3页China Pharmacist

摘　　要：目的:建立HPLC法测定大青根中腺苷和丁香树脂酚葡萄糖苷含量的方法。方法:采用Diamnosil ODS-C_(18)色谱柱(200 mm×4.6 mm,5μm),流动相为乙腈-水梯度洗脱,检测波长为254 nm,流速为1.0 ml·min^(-1),柱温为室温,进样量为10μl。结果:腺苷和丁香树脂酚葡萄糖苷线性范围分别为5～100μg·ml^(-1)(r=0.999 6),5～100μg·ml^(-1)(r=0.999 3);平均回收率分别为98.2%(RSD=1.08%,n=6)和98.4%(RSD=1.18%和n=6);样品平均含量分别为0.081,0.014 mg·g^(-1)。结论:本方法可用于大青根药材的质量控制。Objective：To establish an HPLC method for the simultaneous determination of adenosine and syringaresinol-4-O-β-Dglucopyranoside in the root of Clerodendron cyrtophyllum Turcz.Method：A Diamnosil ODS-C_（18） column（200 mm×4.6 mm,5μm） was applied using a mixture of acetonitrile-water as the mobile phase with gradient elution.The flow rate was 1.0 ml·min（-1） and the column temperature was maintained at the room temperature.The detection wavelength was 254 nm.The sample volume was 10μl.Result： The linear range of adenosine and syringaresinol-4-O-β-D-glucopyranoside was 5-100μg·ml（-1）（r =0.999 6） and 5-100μg·ml（-1）（r=0.9993）,respectively.The average recovery was 98.2%with RSD of 1.08%（n =6） for adenosine and 98.4%with RSD of 1.18%（n =6） for yringaresinol-4-O-p-D-glucopyranoside.Conclusion：The method is accurate and reproducible,which can be used in the quality control of the root of Clerodendron cyrtophyllum Turcz.

关 键 词：大青根 腺苷 丁香树脂酚葡萄糖苷 高效液相色谱法 含量测定 

分 类 号：R927.2[医药卫生—药学]