反相高效液相色谱法测定复方黄藤洗液中蛇床子素的含量  

Content Determination of Osthole in Compound Huangteng Lotions by HPLC

作　　者：朱军[1,2] 陈琴华[2] 李鹏[2] 陈富超[2] 周本宏[1] 

机构地区：[1]武汉大学药学院,武汉430072 [2]湖北医药学院附属东风医院药学部

出　　处：《中国药师》2013年第5期691-692,共2页China Pharmacist

基　　金：湖北省教育厅科研研究计划项目(编号:D20122401;B20122424);湖北省卫生厅青年科技人才项目(编号:QJX2012-45)

摘　　要：目的:建立快速、灵敏的高效液相色谱法测定复方黄藤洗液中蛇床子素含量的方法。方法:采用日本DL-C_(18/)柱(4.6 mm×150 mm,5μm)为色谱柱,甲醇-水(65:35)为流动相,检测波长322 nm,流速为1.0 ml·min^(-1)。结果:蛇床子素在浓度为4～100μg·ml^(-1)范围内有良好的线性关系,r=0.999 3,平均加样回收率为99.76%,RSD为1.59%。结论:本方法操作简单、快速、准确,可用于复方黄藤洗液中蛇床子素的含量测定。Objective： To establish a rapid and sensitive RP-HPLC method for the determination of osthole in compound Huangteng lotions. Method： A DL-CIs column（4.6 mm x 150 ram,5 p.m） was used, the mobile phase consisted of methanol-water （65： 35 ） and the flow rate was 1.0 ml/min. The detection wavelength was 322 nm. Result ： The linear range of osthole was 4-100 μg/ml（ r = 0. 999 3）. The method RSD was l. 42%. The mean extraction recovery was 99.76% with RSD of 1.59%. Conclusion： The method is accurate, sensitive and reliable, and suitable for the determination of osthole in compound Huangteng lotions.

关 键 词：复方黄藤洗液 高效液相色谱法 蛇床子素 

分 类 号：R927.2[医药卫生—药学]