复方藁本内酯微乳的制备和含量测定  

Preparation and Determination of Compound Ligustilide Microemulsions

作　　者：李穆琼[1] 王庆伟[2] 张东旭[1] 徐雪飞[1] 韩晶阔[1] 李晓晔[1] 

机构地区：[1]第四军医大学药学系药物化学教研室,西安710032 [2]第四军医大学唐都医院药剂科

出　　处：《中国药师》2013年第5期693-696,共4页China Pharmacist

摘　　要：目的:制备复方藁本内酯微乳并测定其主要成分藁本内酯和桂皮醛的含量。方法:制备复方藁本内酯微乳,HPLC法测定其主要成分含量。色谱柱为Inertsil ODS-3(250 mm×4.6 mm,5μm)。测定藁本内酯时流动相为乙腈:甲醇:水(10:60:30),柱温为5℃,检测波长为280 nm;测定桂皮醛时流动相为甲醇:水(60:40),柱温为25℃;检测波长为290 nm。流速均为1.0 ml·min^(-1)。结果:藁本内酯在0.50～50.00μg·ml^(-1)(r=0.999 9),桂皮醛在1.30～130.18μg·ml^(-1)(r=0.999 8)范围内线性良好,藁本内酯的回收率为99.65%,RSD为0.97%(n=9);桂皮醛的回收率为99.81%,RSD为0.62%(n=9)。结论:复方藁本内酯微乳制备简单,以HPLC方法测定其主成分含量快速,准确,可靠。Objective：To establish a method for the preparation of compound ligustilide microemulsions and determine ligustilide and cinnamaldehyde in the microemulsions.Method：The compound ligustilide microemulsions were prepared and determined by HPLC.An Inertsil ODS-3 column（250 mm×4.6 mm,5μm）was used.The mobile phase consisted of acetonitrile-methanol -water （10：60：30） for the determination of ligustilide,the detection wavelength and the column temperature was 285 nm and 35℃,respectively. The mobile phase consisted of methanol-water（60：40） for the determination of cinnamaldehyde,the detection wavelength and the column temperature was 290 nm and 25℃,respectively.The flow rate was 1.0 ml·min^（-1）.Result：Ligustilide and cinnamalde-hyde was linear within the range of 0.50-50.00μg·ml^（-1）（r=0.9999）and 1.30-130.18μg·ml^（-1）（r=0.9998）,respectively. The recovery of ligustilide was 99.65%with RSD of 0.97%（n=9）,and that of cinnamaldehyde was 99.81%with RSD of 0.62%（n =9）.Conclusion：The preparation method is simple.The determination method is rapid,accurate and reliable.

关 键 词：复方藁本内酯微乳 藁本内酯 桂皮醛 高效液相色谱法 

分 类 号：R927.2[医药卫生—药学]