具有杀蚜活性的海洋真菌筛选及其活性成分分析  

Screening of marine fungi with bioactivity against Myzus persicae and analysis of its active components

作　　者：陈福龙[1] 王秀芳[1] 陈丹[1] 王新伟[1] 任广伟[1] 

机构地区：[1]中国农业科学院烟草研究所,农业部烟草类作物质量控制重点实验室,青岛266101

出　　处：《植物保护学报》2013年第2期155-159,共5页Journal of Plant Protection

基　　金：农业部烟草类作物质量控制重点开放实验室开放课题(201003);贵州省烟草公司项目(201117)

摘　　要：为筛选可产生杀虫活性代谢物的海洋真菌,评价其在农林害虫防治中的潜在应用价值,从21株海洋真菌中复筛得到对烟蚜有较高生物活性的菌株mfz19,用点滴法测定该菌株对烟蚜的触杀作用,并对其发酵液用乙酸乙酯萃取蒸馏和大孔吸附树脂分离纯化后进行GC-MS检测。菌株mfz 19发酵液的乙酸乙酯提取物浓度为500μL/mL时对烟蚜毒杀作用显著,24 h校正死亡率为92.91%,LC50为91.40μL/mL。杀虫活性提取物为一种混合物,其中1,3-二烯丙基脲的相对含量达到90.40%。研究表明该菌株具有较高的杀蚜活性和开发潜力。In order to screen out tial application, ondary screening the strain mfzl9 marine fungi with insecticidal activity metabolites and evaluate its poten- with bioactivity against Myzus persicae （Sulzer） was obtained after sec- in 21 marine fungal strains. Its bioactivity against M. persicae was determined using con- tact toxicity test. After extracted and distilled with ethyl acetate and purified by macroporous resin, the fermentation broth was detected by GC-MS. Results showed that strain mfz19 had obvious toxicity against M. persicae when the extract concentration of its broth reached 500 μL/mL. The corrected mortality rate after 24 h was 92.91%, and the LCs0 was 91.40 μL/mL. The extract with insecticidal activity was detec- ted as a certain mixture, and the relative content of 1, 3-diallylurea reached 90.40%. This implied that strain mfzl9 might have high insecticidal activity against M. persicae and perfect development potential.

关 键 词：海洋真菌 次生代谢物 烟蚜 生物测定 

分 类 号：S476.1[农业科学—农业昆虫与害虫防治]