胞壁酰二肽对人主动脉内皮细胞炎症因子表达影响的研究  

The effects of muramyl dipeptide on the expression of inflammatory cytokine in human aortic endothelial cells

作　　者：吕青山[1,2] 李峰[1,2] 曹琳[1,2] 徐瑜[1,2] 

机构地区：[1]湖南师范大学第一附属医院老年病科 [2]湖南省人民医院老年病科,湖南长沙410005

出　　处：《海南医学院学报》2013年第7期919-923,共5页Journal of Hainan Medical University

基　　金：中国高校医学期刊临床专项资金项目(112210702)~~

摘　　要：目的:观察胞壁酰二肽对人主动脉内皮细胞数种炎症因子表达的影响。方法:用胞壁酰二肽(MDP)按要求对人主动脉内皮细胞(HAECs)进行处理,运用实时定量RT-PCR以及RT-PCR测定IL-1β,IL-8,MCP-1,IFN-α与IFN-β基因的表达。用Western blot与RT-PCR方法研究MDP影响IFN-α,IFN-β表达的机制。结果:经MDP处理后,HAECs细胞中炎症因子IFN-α,IFN-β,IL-1β,IL-8和MCP-1的表达上调。结论:MDP可以通过上调HAECs细胞炎症因子的表达。Objective： To observe effects of muramyl dipeptide on the expression of inflammatory cytokine in human aortic endothelial cells. Methods. Human aortic endothelial ceils （HAECs） were trea- ted with muramyl dipeptide （MDP） according to the requirements, the gene expression of IL-1β, IL-8, MCP-1 IFN-α and IFN-β were measured by RT-PCR and real time RT-PCR method. RT-PCR and Western blot were used to explore the mechanism of the up-regulated of IFN-β and IFN-β. Results： After muramyl dipeptide （MDP） treatment, the expression of IL-1β, IL-8, MCP-1 IFN-β and IFN-β were up-regulated. Conclusion： MDP can injure endothelial function by up-regulate the expression of inflammatory eytokine.

关 键 词：胞壁酰二肽 人主动脉内皮细胞 炎症因子 

分 类 号：R543[医药卫生—心血管疾病]