高浓度D-二聚体检测时稀释倍数的探讨  

Optimal dilution of high concentration D-dimer in D-dimer assay

作　　者：钟政荣[1] 徐云侠[2] 田万林[1] 宫继勇[1] 刘蕴霞[1] 张伦军[1] 

机构地区：[1]蚌埠医学院第一附属医院检验科,安徽蚌埠233004 [2]蚌埠医学院第一附属医院神经内科,安徽蚌埠233004

出　　处：《蚌埠医学院学报》2013年第4期466-468,478,共4页Journal of Bengbu Medical College

基　　金：安徽省教育厅自然科学研究重点资助项目(KJ2011A205)

摘　　要：目的:分析高浓度D-二聚体(D-D)血浆稀释8倍时的错误结果,探讨高浓度D-D血浆检测时的最适稀释倍数。方法:将D-D标准血浆稀释成连续的6个稀释度进行标准曲线的制备,再将D-D标准血浆和高浓度血浆分别在机内和机外稀释2倍、4倍、8倍,再在定标后的血凝仪上进行检测,D-D报告结果机内模式仪器自动乘以稀释倍数,机外需人工乘以稀释倍数换算,最后比较机内和机外2种稀释模式的结果差异以及各稀释倍数与原液的结果差异。结果:D-D标准曲线呈一"S"型曲线。机内8倍稀释与机外2倍稀释后的D-D结果差异均有统计学意义(P<0.01),但2倍稀释与原液结果差异无统计学意义(P>0.05),2种稀释模式下相同稀释度之间的结果差异亦均无统计学意义(P>0.05)。结论:高浓度D-D血浆稀释倍数愈大,结果差异愈明显,选择能够检出的最小稀释倍数是高浓度D-D检验结果的质量保证。Objective： To analyze the reasons for unreliable outcome resulting from the plasmas with high D-dimer （D-D） concentration diluted to 8 times, and explore the optimal dilution of the plasmas for D-D test. Methods：The D-D standard curve was derived from the six concentration levels of D-D standard plasma by a customary way. The standards and the patient plasma were diluted to 2 times ,4 times and 8 times in a coagulation analyzer or by manual, and then they were analyzed in a calibrated analyzer;The results in the analyzer and by manual were statistically compared. Results ： The standard curve of D-D was similar to the letter ＂S＂. The D-D concentration when the plasma was diluted to 8 times in the analyzer was decreased significantly （P 〈 0.01 ） , compared with that when diluted to 2 times by manual. However, there was not statistical difference between the plasma and its dilution to 2 times or between the same dilutions in both modes （P 〉 0.05 ）. Conclusions：The higher the dilution is, the more obvious the difference will be. Therefore, the plasma with high D-D concentration should be diluted as less times as it can be determined in order to assure the reliability of D-D value.

关 键 词：D-二聚体 稀释 标准曲线 

分 类 号：R446.1[医药卫生—诊断学]