濒危植物皱花细辛的组织培养  

Tissue culture of endangered plant Asarum crispulatum

作　　者：闫晓慧[1] 谈锋[2] 王瑞波[2] 胡世俊[1] 

机构地区：[1]西南林业大学林学院,云南昆明650224 [2]西南大学生命科学学院,重庆400715

出　　处：《广东农业科学》2013年第7期149-151,共3页Guangdong Agricultural Sciences

基　　金：国家自然科学基金(31200265);西南林业大学校重点基金(111123);云南省教育厅重点项目(50117015)

摘　　要：以皱花细辛的根状茎、叶为材料进行组织培养试验。结果表明,根状茎是最理想的外植体。通过不同的激素配比筛选发现,外植体在MS+6-BA2.0 mg/L+NAA0.5 mg/L的培养基中有利于诱导愈伤组织,诱导率可达80%以上;培养基MS+6-BA1.0 mg/L+NAA0.1 mg/L最有利于芽的分化;组培苗最理想的生根培养基为1/2MS+IBA0.5 mg/L,生根率可达90%以上。The rhizome and leaf of Asarum crispulatum, an endangered plant, were used as explants for tissue culture. The results showed that the rhizome of A. crispulatum was better for tissue culture. The effects of different concentration of 6-BA and NAA on the callus and shoot induction were studied. It showed that MS＋6-BA2.0 mg/L＋NAAO.5 mg/L was the best medium for callus induction with the induce rate more than 80% and MS＋6-BA 1.0 mg/L＋NAA 0.1 mg/L was the best medium for shoots induction. The medium of 1/2MS ＋IBA 0.5 mg/L was fit for rooting of the plantlets with the root inducing rate more than 90%.

关 键 词：濒危植物 皱花细辛 组织培养 快速繁殖 

分 类 号：Q943.1[生物学—植物学]