齐墩果酸诱导Kasumi-1细胞凋亡及对AML1-ETO表达的影响  

Effects of oleanolic acid on apoptosis and AML1-ETO expression of kasumi-1 cells

作　　者：张洁[1] 付云[2] 千新来[1] 

机构地区：[1]新乡医学院病理学教研室,河南新乡453003 [2]新乡医学院生物化学与分子生物学教研室,河南新乡453003

出　　处：《中国临床药理学杂志》2013年第5期367-369,共3页The Chinese Journal of Clinical Pharmacology

摘　　要：目的观察齐墩果酸(OA)诱导人急性髓系白血病M2型细胞株Kasumi-1凋亡及对AML1-ETO表达的影响。方法用不同浓度OA作用于人急性髓系白血病M2型Kasumi-1细胞株,四氮唑蓝(MTT)法检测细胞增殖抑制率;Annexin V/PI双染色后,流式细胞仪检测细胞凋亡;Western blot法检测AML1-ETO蛋白表达水平;荧光原位杂交(Fish)检测融合基因AML1-ETO的变化。结果 OA以时间和剂量依赖方式抑制Kasumi-1细胞的增殖,24,48,72 h的IC50分别约为0.58,0.41,0.35μmol.L-1。不同浓度(0.4,0.6,0.9μmol.L-1)OA作用24 h后,细胞凋亡率明显增加,AML1-ETO蛋白表达下调。结论 OA对Kasumi-1细胞的抑制增殖和诱导凋亡作用可能与AML1-ETO的抑制有关。Objective To explore the effects of oleanolic acid （OA） on AML1 - ETO expression and apoptosis of Kasumi - 1 cells. Methods Kasumi - 1 cells were treated with OA of different concentration. The in- hibitory rate was measured by MTT method. The apoptosis rate of Kasumi -1 cells was determined by flow cytometry with Annexin V/PI double staining. The expression of AML1 - ETO protein was detected by West- ern blot and Fish. Results The results showed that oleanlic acid inhibi- ted the proliferation of Kasumi - 1 cells in time - and dose - dependent manners. The 50% growth inhibition （ IC50 ） at 24, 48 and 72 hours were about 0. 58, 0. 41 and 0. 35μmol·L^-1, respectively. The apoptotic rates of Kasumi - 1 cells treated with oleanlic acid （0.4, 0. 6, 0. 9 μmol·L^-1 ） for 24 h were increased significantly, and AML1 - ETO pro- tein expressions were down - regulated in Kasumi - 1 cells treated with oleanlie acid of different concentration. Conclusion It can be conclu- ded that downregulation of AML1 -ETO protein may be involved in oleanlic acidinduced Kasumi- 1 cells apoptosis.

关 键 词：齐墩果酸 KASUMI-1细胞 细胞凋亡 AML1-ETO 

分 类 号：R973[医药卫生—药品] R965.1[医药卫生—药学]