壳聚糖-三聚磷酸酸钠包载促红细胞生成素对大鼠外伤性脑损伤的作用  

Effects of loaded-erythropoietin with chitosan-sodium tripolyphosphate nanoparticles in traumatic brain injury rats

作　　者：刘辉[1] 张永森[1] 赵家鹏[1] 祖向阳[1] 刘鹏宇[1] 杨卫泷[1] 邢俊领[1] 许国栋[1] 

机构地区：[1]新乡医学院第三附属医院神经外科,河南新乡453000

出　　处：《中国临床药理学杂志》2013年第5期376-377,385,共3页The Chinese Journal of Clinical Pharmacology

摘　　要：目的研究壳聚糖和三聚磷酸钠(TPP)包载EPO的纳米粒对受伤性脑损伤(TBI)大鼠的作用。方法用离子交联法,制备壳聚糖(chitosan,CS)-三聚磷酸钠(TPP)/EPO纳米粒,对纳米粒进行表征。TBI大鼠分成4组(每组8只),用PBS、1 mg.mL-1壳聚糖(CS)-TPP 300μL、200 U.mL-1EPO 300μL和CS-TPP/EPO 300μL分别皮下注射,用水迷宫法评价其对脑损伤的作用。结果壳聚糖-三聚磷酸钠/EPO纳米粒的粒径(348±8)nm,EPO的包封率为75.16%,在pH 7.4的释放曲线成典型的双相释放。载EPO纳米粒对TBI大鼠作用明显优于对照组。结论壳聚糖-三聚磷酸钠/EPO纳米粒能显著改善TBI的损伤。Objective To study the protective effects of loaded erythropoietin （EPO） with chitosan/tripolyphosphate nanoparticles in traumatic brain injury （TBI） model. Methods Chitosan （ CS ） - tripolyphosphate （TPP）/EPO nanoparticles were subcutaneous injection in prepared using ionic gelation, and were characterized. TBI model were divided into four groups（all n =8）. PBS, 1 mg · mL^-1 CS -TPP 300 μL, 200 U · mL-1 EPO 300 μL and CS -TPP/EPO 300 μL nanoparticles were iv 333I rats and the efficacy was evaluated using Morris water maze test. Re- suits The size of CS - TPP/EPO nanoparticles was （348 ± 8） nm, which was further verified by scanning electron microscope （SEM）. EPO loading efficiency of CS/TPP nanoparticles was 75. 16%, and the EPO release of nanoparticles was bi - phase release at pH 7.4. The efficacy of CS -TP/EPO in TBI rats was better than control group. Conclusion CS - TPP/EPO could

关 键 词：壳聚糖纳米粒 促红细胞生成素 外伤性脑损伤 

分 类 号：R973.5[医药卫生—药品] R742.89[医药卫生—药学]