白蔹甲醇提取物对骨髓瘤细胞SP20增殖及凋亡的影响  

Influence of Methanol Extract of Radix Ampelopsis on Proliferation of Myeloma Cell Line SP20

作　　者：张寒[1] 梁晓莉 贾敏[1] 孙艳平[1] 汪兴军 

机构地区：[1]西安医学院,陕西西安710021 [2]陕西步长制药有限公司,陕西咸阳712000

出　　处：《中药新药与临床药理》2013年第3期239-241,共3页Traditional Chinese Drug Research and Clinical Pharmacology

基　　金：陕西省教育厅自然科学专项基金(2010JK814)

摘　　要：目的研究白蔹甲醇提取物不同极性萃取部位对骨髓瘤细胞SP20增殖及凋亡的影响。方法体外培养骨髓瘤SP20细胞,光学显微镜观察加入白蔹甲醇提取物不同萃取部位后的细胞形态的变化;MTT法检测白蔹甲醇提取物不同极性萃取部位对骨髓瘤细胞增殖的影响。结果光学显微镜观察到给药后骨髓瘤SP20细胞出现典型的细胞凋亡形态改变。药物作用48 h后,5-氟尿嘧啶组、氯仿组、乙醚组、乙酸乙酯组对细胞增殖均有抑制作用,与溶剂空白组比较,差异有统计学意义(P<0.05,P<0.01);正丁醇组抑制率最小,与溶剂空白组比较,差异无统计学意义(P>0.05)。白蔹甲醇提取物不同极性萃取部位对骨髓瘤细胞存活的抑制率,从大到小依次是:乙酸乙酯组>乙醚组>氯仿组。结论白蔹甲醇提取物对体外培养的骨髓瘤细胞的生长有明显的抑制作用,并促其凋亡。Objective To study the influence of methanol extract of Radix Ampelopsis with different polarity on myeloma cell line SP20. Methods After culturing myeloma cells SP20 in vitro, the cell morphological changes were observed under optical microscope, and influence of methanol extraction parts of Radix Ampelopsis with different polarity on SP20 myeloma cells proliferation was detected by MTT assay. Results After co-cultured with the different polar extraction parts of Radix Ampelopsis for 48 hours, SP20 cells showed apoptotic morphological changes under optical microscope. Chloroform, 5-fluorouracil, ethyl ether and ethyl acetate part had inhibition on the proliferation of SP20 （P 〈 0.05, P 〈 0.01）, and normal butanol part had the lowest inhibition ratio （P 〉 0.05） compared with the solvent blank group. Inhibition ratio of ethyl acetate, ethyl ether and chloroform parts of Radix Ampelopsis was in decreasing sequence on the survival of SP20. Conclusion Radix Ampelopsis methanol extract can significantly inhibit the growth of SP20 myeloma cells and promote the apoptosis of SP20.

关 键 词：白蔹甲醇提取物 骨髓瘤细胞 抗肿瘤 

分 类 号：R285.5[医药卫生—中药学]