人工种植金银花中绿原酸含量测定和提取工艺研究  

Chlorogenic acid content determination and extraction technology research for artificial planting honeysuckle

作　　者：梁萱[1] 赵建军[2,3] 梁永锋[3] 

机构地区：[1]南昌大学药学系,南昌330031 [2]宁夏隆德西北中药材有限责任公司,隆德756300 [3]宁夏师范学院,固原756000

出　　处：《西北药学杂志》2013年第3期228-230,共3页Northwest Pharmaceutical Journal

基　　金：宁夏科技攻关项目资助(编号:NKJ2010-168-177)

摘　　要：目的测定人工种植金银花中绿原酸的含量和最佳提取工艺。方法采用紫外可见光分光光度法测绿原酸的含量,通过单因素实验和正交实验,优化微波辐射提取金银花中绿原酸的最佳工艺。结果人工种植金银花绿原酸含量为6.40%,最佳提取工艺条件是:微波辐射功率450W,液物料比1∶25,乙醇体积分数70%,微波辐射时间45min,提取溶剂的pH为7。结论人工种植金银花中绿原酸含量较高,完全满足药用。Objective To determine the chlorogenic acid content and to optimize the extraction technology for artificial planting hon- eysuckle. Method The content of chiorogenic acid was determined by an ultraviolet visible spectrophotometric method. The micro- wave assisted extraction of chlorogenic acid in Flos Lonicerae was optimized by a single factor test and an orthogonal experiment. Results Chlorogenic acid content in artificial planting honeysuckle was 6.40 %, the optimal extraction conditions were：microwave power 450 W, liquid and material ratio 1 ： 25 ,volume fraction of ethanol 70 %, microwave irradiation time 45 min, solvent pH = 7. Conclusion Chlorogenic acid content in artificial planting honeysuckle is high,and fully meets the medicinal application.

关 键 词：人工种植 金银花 绿原酸 含量 提取 

分 类 号：R282[医药卫生—中药学]