HPLC法测定野生与栽培肿节风中迷迭香酸的含量  

Determination of romarinic acid in wild and cultivated Sarcandra glabra by HPLC

作　　者：朱丹[1] 农志欢[1] 黄兴振[1] 巫玲玲[1] 蒋伟哲[1] 付书婕[1] 

机构地区：[1]广西医科大学,南宁530021

出　　处：《西北药学杂志》2013年第3期239-241,共3页Northwest Pharmaceutical Journal

基　　金：南宁市科学研究与技术开发计划(编号:201109054C)

摘　　要：目的建立野生与栽培肿节风中迷迭香酸含量的测定方法,分析比较肿节风不同部位中迷迭香酸含量的差异。方法采用HPLC法,固定相为Phenomenex C18色谱柱(250mm×4.6mm,4μm);流动相为乙腈-1mL.L-1磷酸溶液(25∶75);流速为1.0mL.min-1,柱温为40℃,检测波长为342nm。结果野生肿节风根、茎、叶中迷迭香酸的平均含量分别为0.25%,0.42%和1.43%,栽培肿节风根、茎、叶中迷迭香酸的平均含量分别为0.29%,0.49%和1.50%。结论肿节风药材根、茎、叶3个部位中的迷迭香酸含量差异较大,叶中含量最高,茎中其次,根中最低。相同部位比较,栽培肿节风较野生的迷迭香酸含量高。Objective To determine the content of romarinic acid in the roots, stems and leaves of wild and cultivated Sarcandra glabra by HPLC method. Method Phenomenex C18 column （250 mm）〈 4.6 ram, 4 μm） was used, the mobile phase was acetonitrile- 1 mL · L-1 phosphoric acid （25 ： 75） ,and the flow rate was 1.0 mL · min-1. The column temperature was set at 40 ℃ ,the detection wavelength was 342 nm,and the injection volume was 5 μL. Result The average content of rosmarinic acid in roots,stems and leaves of the wild was 0.25% ,0.42% and 1.43% ,respectively,and average content of rosmarinic acid in the cultivated was 0. 29% ,0.49% and 1.50 % ,respectively. Conclusion The content of rosmarinic acid is significantly different in roots, stems and leaves. The culti- vated showed a higher content of rosmarinic acid than the wild in the same organ.

关 键 词：肿节风 野生 栽培 迷迭香酸 

分 类 号：R282[医药卫生—中药学]