狼紫草水提物对小鼠体表溃疡模型组织中环氧酶-2表达的影响  

Effects of aqueous extract from Lycopsis orientalis L on skin ulcer tissue and cyclooxygenase-2 expression in mice

作　　者：孙文晓[1,2] 杨华[2] 卢小岚[2] 张海港[2] 

机构地区：[1]华中科技大学同济医学院附属梨园医院骨科,湖北武汉430077 [2]第三军医大学药物研究所与药剂学教研室,重庆400038

出　　处：《中国医院药学杂志》2013年第10期757-759,共3页Chinese Journal of Hospital Pharmacy

基　　金：国家自然科学基金资助项目(编号:30472034);湖北省自然科学基金项目(编号:2010CDB07501)

摘　　要：目的:研究狼紫草水提物对小鼠皮肤溃疡组织环氧酶-2(cyclooxygenase-2,COX-2)表达的影响。方法:煎煮法制备狼紫草水提物,醋酸化学刺激法制备小鼠体表溃疡模型。分别采用Western blotting和RT-PCR法测定局部皮肤溃疡组织中环氧酶-2蛋白及其mRNA表达情况。结果:正常小鼠皮肤组织中几乎无COX-2表达,模型组小鼠体表溃疡组织中COX-2蛋白及其mRNA的表达均显著增加,应用高剂量(40g.kg-1)和中剂量(20g.kg-1)狼紫草水提物治疗后,二者表达水平明显降低。结论:狼紫草水提物对COX-2有明显的干预作用,可预见其抗炎镇痛作用有较好应用前景。OBJECTIVE To explore the effects of aqueous extract from Lycopsis orientalis L.（AELO） on the expression of COX-2 in skin ulcer tissue of mice.METHODS AELO was prepared with decocting method and the skin ulcer model was induced by acetic acid chemical stimulus.The expression of cyclooxygenase（COX）-2 protein and mRNA were detected with Western blotting and RT-PCR technique respectively.RESULTS The expressions of COX-2 protein and mRNA obviously increased in skin ulcer tissue as compared with those of normal control group.Nevertheless,after the administration of AELO（40 or 20 g·kg-1）,their expressions decreased significantly.CONCLUSION AELO can significantly down-regulate the expression of COX-2 protein and mRNA in skin ulcer tissue.

关 键 词：狼紫草水提物 体表溃疡 前列腺素E2 环氧酶-2 

分 类 号：R966[医药卫生—药理学]