普通小麦籽粒Tamyb10基因等位变异的分子检测  

Allelic Variations of Tamyb10 Gene Controlling Grain Color of Bread Wheat(Triticum aestivum L.)

作　　者：陈杰[1] 陈锋[1] 詹克慧[1] 刘宝龙[2] 崔党群[1] 

机构地区：[1]河南农业大学农学院/河南省粮食作物生理生态与遗传改良国家重点实验室培育基地,河南郑州450002 [2]中国科学院西北高原生物研究所,青海西宁810008

出　　处：《麦类作物学报》2013年第2期224-229,共6页Journal of Triticeae Crops

基　　金：国家重点基础研究发展计划(973计划)项目(2009CB118301);国家科技支撑计划项目(2011BAD07B01);河南省高校青年骨干教师资助计划项目(2011GGJS-044)

摘　　要：小麦Tamyb10基因决定着种皮颜色,同时对穗发芽也具有一定影响。为了明确小麦种质的Tamyb10基因等位变异情况,以122份普通小麦品种(系)为材料,开发了能够直接检测B组染色体上Tamyb10基因等位变异的共显性标记,并利用该标记及先前已报道的Tamyb10基因功能标记分别对参试小麦品种中3A、3B和3D染色体上Tamyb10基因位点上的等位变异类型进行了检测。结果发现,参试材料中上述每一位点均发现有2种等位变异类型,共有8种基因型组合。其中,拥有Tamyb10-A1a/Tamyb10-B1a/Tamyb10-D1a基因型组合的小麦种皮表现为白色,共有51份,占供试材料总数的41.8%,其余基因型组合种皮均表现红色,分别为Tamyb10-A1b/Tamyb10-B1a/Tamyb10-D1a、Tamyb10-A1a/Tamyb10-B1b/Tamyb10-D1a、Tamyb10-A1a/Tamyb10-B1a/Tamyb10-D1b、Tamyb10-A1b/Tamyb10-B1b/Tamyb10-D1a、Tamyb10-A1b/Tamyb10-B1a/Tamyb10-D1b、Tamyb10-A1a/Tamyb10-B1b/Tamyb10-D1b和Tamyb10-A1b/Tamyb10-B1b/Tamyb10-D1b,这些基因型材料共有71份,占供试材料总数的58.2%。说明当Tamyb10基因在三个位点均为野生型时,种皮颜色为白色;而当任何一个位点发生突变时种皮颜色均表现为红色。The Tamyb10 genes play an important role for controlling the grain color and also have some influence on preharvest sprouting in bread wheat.In this study,the codominant marker T3B-2 was developed for detection of Tamyb10-B1 allele in bread wheat.The new marker T3B-2 and the Tamyb10 functional markers reported previously were used to identify allelic variation on the Tamyb10-A1,Tamyb10-B1 and Tamyb10-D1 loci in bread wheat varieties or advanced lines surveyed,respectively.The results showed that there were two haplotypes on each Tamyb10 locus and eight allelic combinations of Tamyb10 were totally found in bread wheat surveyed.Of them,Tamyb10-A1a/ Tamyb10-B1a/ Tamyb10-D1a possessing 41.8% of the total number of cultivars surveyed show the white grain color and remaining seven allelic combinations accounting for 58.2% show red grain color,i.e.Tamyb10-A1b/ Tamyb10-B1a/ Tamyb10-D1a,Tamyb10-A1a/ Tamyb10-B1b/ Tamyb10-D1a,Tamyb10-A1a/ Tamyb10-B1a/ Tamyb10-D1b,Tamyb10-A1b/ Tamyb10-B1b/ Tamyb10-D1a,Tamyb10-A1b/ Tamyb10-B1a/ Tamyb10-D1b,Tamyb10-A1a/ Tamyb10-B1b/ Tamyb10-D1b and Tamyb10-A1b/ Tamyb10-B1b/ Tamyb10-D1b.It suggests that cultivars with wild-type Tamyb10 at three loci show white grain color and cultivars with at least one mutant of Tamyb10 at three loci show red grain color.This study could provide useful information for screening relatively superior germplasms.

关 键 词：普通小麦 Tamyb10基因 种皮颜色 功能标记 

分 类 号：S512.1[农业科学—作物学] S330