棕色脂肪干细胞与白色脂肪干细胞移植对心肌梗死大鼠心功能的影响  

Effects of brown adipose-derived mesenchymal stem cells and white adipose-derived mesenchymal stem cells transplantation on cardiac function of rat with myocardial infarction

作　　者：石金鑫[1] 刘剑锋[1] 王海滨[2] 朱平[1] 

机构地区：[1]解放军总医院南楼心一科,北京100853 [2]军事医学科学院基础医学研究所前沿交叉学科研究室,北京100850

出　　处：《中国医药导报》2013年第15期18-21,共4页China Medical Herald

基　　金：国家自然科学基金青年基金资助项目(编号81101160)

摘　　要：目的对比研究棕色脂肪干细胞(BADSCs)与白色脂肪干细胞(ADSCs)治疗急性心肌梗死大鼠的效果。方法采用酶消化法分别分离大鼠腹股沟脂肪组织及肩胛骨下脂肪组织,获得ADSCs与BADSCs,并进行流式分析与多谱系分化鉴定。30只雄性SD大鼠随机分为PBS对照组、ADSCs移植组及BADSCs移植组,建立急性心肌梗死模型,将各组对应细胞直接注入大鼠心肌梗死边缘区。移植后4周进行心功能检测并利用组织学检测移植细胞的体内分化与血管化作用。结果术后4周,与PBS对照组相比,ADSCs以及BADSCs移植组大鼠心功能均显著提高(P<0.05),且纤维化程度显著减弱(P<0.05)。免疫荧光结果表明,移植的BADSCs成心肌分化数量显著高于ADSCs。免疫组化结果显示,ADSCs移植组以及BADSCs移植组大鼠心肌梗死区血管密度均较PBS对照组显著增加(P<0.05),ADSCs移植组血管密度显著高于BADSCs移植组(P<0.05)。结论不同来源脂肪干细胞移植均具有改善心肌梗死患者心功能的作用,虽然BADSCs体内促血管化作用不如ADSCs,但其具有明显的心肌分化能力,可有效改善心脏功能。Objective To compare the therapeutic effects of brown adipose-derived mesenchymal stem cells（BADSCs） and white adipose-derived mesenchymal stem cells（ADSCs） in rats with acute myocardial infarction.Methods The BADSCs and ADSCs were prepared by enzyme digestion from brown and white adipose tissues isolated from rat shoul der and inguinal regions,respectively.The stem cells were analyzed by flow cytometry and multipotential differentia tion ability.30 rats were randomly divided into PBS control group,ADSCs transplantation group and BADSCs trans plantation group,then the acute myocardial infarction model was established.The BADSCs or ADSCs were injected in to the edge area of myocardial infarction respectively,the PBS was injected as control.Detailed histological analysis and echocardiography were used to determine the cardiac function and differentiation,vascularization effects of trans plantation cells after 4 weeks of transplantation.Results Compared with PBS control group,the heart function improved significantly with decreased fibrosis formation in the BADSCs and ADSCs transplantation at 4 weeks after transplantation（P 0.05）.Immunofluorescence result showed that the cardiac-differentiation ability of BADSCs was much higher than that of ADSCs in vivo.Immunohistochemical results showed that compared with the PBS control group,the micro-vessel density in scar areas increased significantly in the ADSCs transplantation group and BADSCs transplantation group（P 0.05）,and the ADSCs groups increased most（P 0.05）.Conclusion Both transplantation of BADSCs and ADSCs can enhance the cardiac function of myocardial infarction.Although compared with ADSCs,BADSCs has a lower ability of vascularization,but it can improve the heart performance through the high potential of cardiac-differentiation.

关 键 词：脂肪干细胞 急性心肌梗死 移植 

分 类 号：R542.22[医药卫生—心血管疾病]